Effect Of Fermentation Time On Pyridoxine And Biotin Production By Lactic Acid Bacteria Isolates From Fermented Pentaclethra Macrophylla Benth :- Azubuike, Clement K
ABSTRACT
This research studied the effect of fermentation
on the production of pyridoxine and biotin by Lactic Acid Bacteria from
fermented Pentaclethra macrophylla (Ugba). Locally produced sampls offermented
Pentaclethra macrophylla were bought from Ahiaeke market, Umuahia, Abia State.
Sixteen LAB isolates were found to be Gram positive, non-spore formers and
catalase negative after plating on Ml 7 and MRS (de Man Rogosa and Sharpe)
agar. Four LAB species were isolated from Pentaclethra macrophylla ofwhich were
Lactobacillusfermentum, Lactobacillusplantarum, Streptococcus lactis and
Leuconostoc mesenteroides based on morphological, biochemical and physiological
characteristics. The effect offermentation time on vitamin B6 and B7 production
by the identified LAB isolates using African Oil Bean Seed (AOBS) broth medium
was studied and the vitamin production was determined by High Performance
Liquid Chromatography (HPLC). The vitamin Be and B7 content of the fermented
Pentaclethra macrophylla was increased by 99.89% (from 0.0052 to 0.6137 mg/ml)
and 6.45% (0.2029 to 0.2198 mg/ml) respectively. Evaluation ofthe effect
offermentation time presented interesting findings as there was an increase in
the content ofpyridoxine initiated by LAB fermentation. However, the increase
in biotin content was relatively low compared to that of pyridoxine and may be
attributed to its utilization as a growth requirement by some LAB. Hence,
optimization offermentation time and a better selection of microbial species as
part of the starter cultures will be an advancement in the production of
fermented products with elevated levels ofthese essential vitamins rather than
traditional starters, which consume these vitamins.
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AZUBUIKE (2026). Effect Of Fermentation Time On Pyridoxine And Biotin Production By Lactic Acid Bacteria Isolates From Fermented Pentaclethra Macrophylla Benth :- Azubuike, Clement K. Repository.mouau.edu.ng: Retrieved Feb 18, 2026, from https://repository.mouau.edu.ng/work/view/effect-of-fermentation-time-on-pyridoxine-and-biotin-production-by-lactic-acid-bacteria-isolates-from-fermented-pentaclethra-macrophylla-benth-azubuike-clement-k-7-2
AZUBUIKE. "Effect Of Fermentation Time On Pyridoxine And Biotin Production By Lactic Acid Bacteria Isolates From Fermented Pentaclethra Macrophylla Benth :- Azubuike, Clement K" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 17 Feb. 2026, https://repository.mouau.edu.ng/work/view/effect-of-fermentation-time-on-pyridoxine-and-biotin-production-by-lactic-acid-bacteria-isolates-from-fermented-pentaclethra-macrophylla-benth-azubuike-clement-k-7-2. Accessed 18 Feb. 2026.
AZUBUIKE. "Effect Of Fermentation Time On Pyridoxine And Biotin Production By Lactic Acid Bacteria Isolates From Fermented Pentaclethra Macrophylla Benth :- Azubuike, Clement K". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 17 Feb. 2026. Web. 18 Feb. 2026. < https://repository.mouau.edu.ng/work/view/effect-of-fermentation-time-on-pyridoxine-and-biotin-production-by-lactic-acid-bacteria-isolates-from-fermented-pentaclethra-macrophylla-benth-azubuike-clement-k-7-2 >.
AZUBUIKE. "Effect Of Fermentation Time On Pyridoxine And Biotin Production By Lactic Acid Bacteria Isolates From Fermented Pentaclethra Macrophylla Benth :- Azubuike, Clement K" Repository.mouau.edu.ng (2026). Accessed 18 Feb. 2026. https://repository.mouau.edu.ng/work/view/effect-of-fermentation-time-on-pyridoxine-and-biotin-production-by-lactic-acid-bacteria-isolates-from-fermented-pentaclethra-macrophylla-benth-azubuike-clement-k-7-2