Screening And Isolation Of Amylolytic Bacteria Involved In Biogas Production

ABSTRACT

The Isolation and characterization of amylolytic bacterial strains with hydrolytic profile during anaerobic digestion of the mixture of cow rumen, dried cassava peels and poultry droppings was the subject of this research. Hydrolysis is important to biogas production as it involves breakdown of polysaccharides present in substrates. The experiment lasted for 30 days using a 10 litre scale bio-digester. Hydrolytic amylase producing bacteria isolated were Micrococcus luteus, Pseudomonas aeruginosa, Klebsiella oxytoca, Aspergillus spp, and Bacillus subtilis. The total anaerobic plate count ranged from 2.57cfu/g to 3.53cfu/g and the observed pH ranged from 5.12 to 6.19. The study reveals that the mixture of cassava peels, poultry droppings and cow rumen has the potential of producing biogas. In conclusion, further investigation of hydrolytic groups of bacteria beyond 30 days of anaerobic retention period should be investigated.