Isolation And Identification Of Cellulolytic Bacteria Involved In Biogas Production.

ABSTRACT

 This study focused on the isolation and identification of cellulolytic bacteria involved in biogas production using cassava peels (CP) and chicken dropping (CD) as substrates in the ratio of 1: I with the water. Variation of pH and temperature during biogas production were determined. The digester were stirred twice daily to avoid scum formation in the digester and to allow for easy escape of the gas produced. Evaluation of amount of biogas produced by the substrates was studied. The retention time used for this experiment was 42 days during which the daily internal temperature reading was taken in order to determine the temperature variation. Standard microbiological methods were used for the isolation and wastes substrates for biogas production. Four cellulolytic bacteria were isolated they are Bacillus, Micrococcus, P,seudonionas and Kiebsiella species. The total anaerobic plate count ranged from 0.59 X 106 cfu/ml to 1.2 X1O6 cfu/ml. the observed pH range were 5.12- 6.19. In conclusion, the study reveals that cassava peels and chicken droppings has potential of producing biogas.