Beta-.Lactamase Production By Methicillin Resistant Staphylococcus Aureus Isolated From Stool And Urine Samples Of Healthy Students Of Michael Okpara University Of Agriculture, Umudike.

ABSTRACT

Evaluation of beta lactamase production potential of Staphylococcus aureus isolates obtained from a total of 100 stool and urine samples was carried out in this study. Samples were streaked directly on manitol salt agar for isolation of S. aureus A total of 16 and 17 S. aureus Isolate we're obtained from the 50 sample of urine and stool samples studied representing 32.0% and 34.0% respectively. This gave a combined total of 33 5. aureus Isolates from the 100 samples studied. Ofloxacin, levofloxacin, and Ciprofloxacin we're the most potent antibiotics giving 81.8%, 81.8% and 72.7% susceptibility values respectively. Gentamicin and Erythromycin also had 60.6% and 33.3% susceptibility values whereas the Isolates show complete (100%) resistance to Augmantin and ceffazidime. This study revealed that 13 out of the total 33 Isolate were Methicillin resistance while the remaining 20(60.6%) were Methicillin sensitive. Similarly, 23(69.7%) of the total isolate screened by the acidometric method tested positive for betalactamase production. This study shows that 69.7% student tested for S. aureus are resistance to beta-lactam drugs.