Isolation And Antibiotic Sensitivity Test Of Pathogenic Bacteria From Ready-To-Eat Food Sold In Michael Okpara University Of Agriculture, Umudike:- Samuel, Ebuka P.
ABSTRACT
Today the issue of food safety is a global
problem that gets main concern in setting public health policy. The eruption of
diseases caused by food contamination occurs in places where sanitation and
hygiene conditions are generally poor. This study was conducted to isolate and
determine the antibiogram of bacteria pathogens in ready-to-eat foods sold in
MOUAU campus. Four ready-to-eat food samples were collected from different
vendors within the campus and analyzed immediately through serial dilution,
inoculation, incubation, subculture, microscopy and biochemical to confirm the
isolated organisms. Antibiotics sensitivity test was also carried out to
determine antibiotic sensitivity on the isolated organisms. A total of four
pathogenic bacteria were isolated: Staphylococcus aureus (40%), Salmonella
(17.5%), Escherichia coli (e0%) and Shigella (12.5%). Eight different
antibiotic were used to carry out antibiogram of the isolates as the inhibition
zone was measured in millimeter: Oflovacin (OFX), Amoxil (AMX), Gentamycin
(CN), Augmentin (AUG), Ciprofloxacin (CPX), Chloramphenicol (CH), Ampiclox
(APX) and Streptomycin (S). (AUG) and (CPX) had high effect on Salmonella and
Escherichia coli and was less on Staphylococcus aureus while (OFX) and (S) were
very effective on Staphylococcus aureus and less effective on Escherichia coli
and Salmonella. This shows the poor hygiene level of the food vendors as
majority ofthe food vendors lacks western education. These vendors have to
receive education and training on food hvgiene to improve the safety of foods
in MOUAU and thereby heighten the safety of consumers.
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EBUKA, P (2024). Isolation And Antibiotic Sensitivity Test Of Pathogenic Bacteria From Ready-To-Eat Food Sold In Michael Okpara University Of Agriculture, Umudike:- Samuel, Ebuka P.. Repository.mouau.edu.ng: Retrieved May 01, 2024, from https://repository.mouau.edu.ng/work/view/isolation-and-antibiotic-sensitivity-test-of-pathogenic-bacteria-from-ready-to-eat-food-sold-in-michael-okpara-university-of-agriculture-umudike-samuel-ebuka-p-7-2
PROMISE, EBUKA. "Isolation And Antibiotic Sensitivity Test Of Pathogenic Bacteria From Ready-To-Eat Food Sold In Michael Okpara University Of Agriculture, Umudike:- Samuel, Ebuka P." Repository.mouau.edu.ng. Repository.mouau.edu.ng, 18 Apr. 2024, https://repository.mouau.edu.ng/work/view/isolation-and-antibiotic-sensitivity-test-of-pathogenic-bacteria-from-ready-to-eat-food-sold-in-michael-okpara-university-of-agriculture-umudike-samuel-ebuka-p-7-2. Accessed 01 May. 2024.
PROMISE, EBUKA. "Isolation And Antibiotic Sensitivity Test Of Pathogenic Bacteria From Ready-To-Eat Food Sold In Michael Okpara University Of Agriculture, Umudike:- Samuel, Ebuka P.". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 18 Apr. 2024. Web. 01 May. 2024. < https://repository.mouau.edu.ng/work/view/isolation-and-antibiotic-sensitivity-test-of-pathogenic-bacteria-from-ready-to-eat-food-sold-in-michael-okpara-university-of-agriculture-umudike-samuel-ebuka-p-7-2 >.
PROMISE, EBUKA. "Isolation And Antibiotic Sensitivity Test Of Pathogenic Bacteria From Ready-To-Eat Food Sold In Michael Okpara University Of Agriculture, Umudike:- Samuel, Ebuka P." Repository.mouau.edu.ng (2024). Accessed 01 May. 2024. https://repository.mouau.edu.ng/work/view/isolation-and-antibiotic-sensitivity-test-of-pathogenic-bacteria-from-ready-to-eat-food-sold-in-michael-okpara-university-of-agriculture-umudike-samuel-ebuka-p-7-2