Effectof Processing On Hemagglutinin Activity Of Lectin Extract From Selected Green Vegetables (Vernonia Amygdalina, Gnetum Africanum, Petrocarpus Soyauxil And Telifera Occidentalis)

EDWARD QUEEN AKUBUEZE | 59 pages (12142 words) | Projects

ABSTRACT

Lectins are carbohydrate binding proteins present in most plants. They are toxic, inflammatory, and resistant to cooking and digestive enzymes and present in many of our foods. In this study, lectins were extracted from Okazi leaf (Gnetum africanum), Bitter leaf (Venonia amygdalina), Fluted pumpkin leaf (Telferia occidentalis), and Oha leaf (Pterocarpus soyauxii). Partial purification of the lectin extracts was achieved by precipitation with ammonium sulphate and the extract was tested for hemagglutinin activity using 4% suspension of human erythrocytes in lectin buffer. Extracts from the above selected vegetables agglutinated all the blood groups (A, B, AB and 0) suggesting the presence of non blood type specific lectin in the vegetables. Since agglutination readily occurred without the need for proteolytic enzyme or addition of metal ion, the crude extract could be regarded as complete lectin. The hemagglutinin activity of the vegetables lectin were found decrease when exposed to processing methods ( oven drying and moist heating). Also the extract decreased with increase in incubation temperature. Strong and moderate agglutination was observed in non incubated sample and samples incubated at 10C and 30C while agglutinations was devoid when subjected to temperature of 70C and 100C, suggesting that lectin from these vegetables are heat labile and can be deactivated by moist or dry heating.

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APA

EDWARD, A (2022). Effectof Processing On Hemagglutinin Activity Of Lectin Extract From Selected Green Vegetables (Vernonia Amygdalina, Gnetum Africanum, Petrocarpus Soyauxil And Telifera Occidentalis). Repository.mouau.edu.ng: Retrieved Feb 07, 2023, from https://repository.mouau.edu.ng/work/view/effectof-processing-on-hemagglutinin-activity-of-lectin-extract-from-selected-green-vegetables-vernonia-amygdalina-gnetum-africanum-petrocarpus-soyauxil-and-telifera-occidentalis-7-2

MLA 8th

AKUBUEZE, EDWARD. "Effectof Processing On Hemagglutinin Activity Of Lectin Extract From Selected Green Vegetables (Vernonia Amygdalina, Gnetum Africanum, Petrocarpus Soyauxil And Telifera Occidentalis)" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 22 Nov. 2022, https://repository.mouau.edu.ng/work/view/effectof-processing-on-hemagglutinin-activity-of-lectin-extract-from-selected-green-vegetables-vernonia-amygdalina-gnetum-africanum-petrocarpus-soyauxil-and-telifera-occidentalis-7-2. Accessed 07 Feb. 2023.

MLA7

AKUBUEZE, EDWARD. "Effectof Processing On Hemagglutinin Activity Of Lectin Extract From Selected Green Vegetables (Vernonia Amygdalina, Gnetum Africanum, Petrocarpus Soyauxil And Telifera Occidentalis)". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 22 Nov. 2022. Web. 07 Feb. 2023. < https://repository.mouau.edu.ng/work/view/effectof-processing-on-hemagglutinin-activity-of-lectin-extract-from-selected-green-vegetables-vernonia-amygdalina-gnetum-africanum-petrocarpus-soyauxil-and-telifera-occidentalis-7-2 >.

Chicago

AKUBUEZE, EDWARD. "Effectof Processing On Hemagglutinin Activity Of Lectin Extract From Selected Green Vegetables (Vernonia Amygdalina, Gnetum Africanum, Petrocarpus Soyauxil And Telifera Occidentalis)" Repository.mouau.edu.ng (2022). Accessed 07 Feb. 2023. https://repository.mouau.edu.ng/work/view/effectof-processing-on-hemagglutinin-activity-of-lectin-extract-from-selected-green-vegetables-vernonia-amygdalina-gnetum-africanum-petrocarpus-soyauxil-and-telifera-occidentalis-7-2

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