Investigation Of Effect Of A Combine Ethanol. Extract Of Andrographispaniculata, Mucuna Pruriens And Ficus Stir (Amficus) On Biochemical Markers Implicated In Diabetes Pathogensis:- Chima Ikechukwu P

ABSTRACT xi This study was carried out Io investigate the effect of a combine ethanol extract ofAndrographis Panicidata, Mticima Pruriens and Ficus Fur {Amficus) on biochemical markers Implicated In diabetes pathogensis. The acute toxicity effect of Amficus was evaluated with twenty-one (21) male albino rats while forty-two (42) male albino rats were used for the evaluation of the rcnoprotective effect of Amficus on alloxan-induced diabetic rats. The forty-two rats were randomly selected into seven groups containing equal number of rats (n = 6). Group 1 was the normal control that received 2 mL/kg normal saline and groups 2-6 were alloxan-induced diabetic rats. The rats were induced diabetic by the intraperitoneal administration of a single dose (160 mg/kg) of alloxan dissolved in normal saline and allowed to stay for 72 h for onset of diabetes. Alloxan-induced rats having blood glucose level above 200 mg/dl were considered diabetic and used for the study. The diabetic induced rats were classified as diabetic control, diabetic rats + 3 mg/kg Glibenclamidc. diabetic rats + 200 mg/kg AMFICUS, diabetic rats + 400 mg/kg AMFICUS, and diabetic rats + 800 mg/kg AMFICUS. The groups 7 rats served as AMFICUS control that received 800 mg/kg AMF1CI IS only. The study was conducted following standard laboratory procedures. The results of the acute toxicity study of AMFICUS indicated that it has LD50 above 5000 mg/kg. The results of renaprotcclive study of AMFICUS indicated • that the diabetic control rats exhibited significantly (P<0.05) elevated serum urea, creatinine, sodium, potassium, chloride and bicarbonate ion concentrations compared with the normal • control and rats administered 800 mg/kg AMFICUS only, respectively. The alterations in the serum urea, creatinine, sodium, potassium, chloride, and bicarbonate ion concentrations were significantly reversed in the diabetic rats treated with different doses of AMFICUS and Glibenclamidc respectively compared with the normal control. The renal sections of diabetic control rats and diabetic rats treated with 200 and 400 mg/kg AMFICUS respectively, displayed multifocal areas of tubular degenerations and necrosis relative to the renal histomorphology of the normal control. Contrarily. the renal histomorphology of diabetic rats treated with 800 mg/kg AMFICUS. and rats administered 800 mg/kg AMFICUS only respectively showed normal histomorphology compared to the diabetic control. The findings from this study show that AMI-ICUS possesses rcnoprotective el feet against renal disorders associated with alloxaninduced diabetes in rats and suggest it could play a critical role in the management of diabetes.