Evaluation Of Chemical Constituents And In Vitro Antiinflammatory Property Of The Essential Oils Of Hibiscus Sabdariffa Seeds:-Chukwuezi, Victoria C

ABSTRACT x Anti-inflammatory properties play major roles in counteracting the deleterious effects of chronic inflammation in biological systems; and the anti-inflammatory potentials of plants arid plant parts are related to the phytoconstituents inherent in them. This study was therefore aimed at evaluating some chemical components and anti-inflammatory property of the essential oil of Hibiscus sabdariffa seeds (EOHSS) using anti-lipoxygenase activity spectrophotometric assay. The essentials oil of Hibiscus sabdariffa seeds (HSS) were subjected to phytochemical analysis using gas chromatography coupled to frame ion detector (FID) and mass spectrometer (MS) the total phenols, total flavonoids and the anti-inflammatory property was examined using the antilipoxygenase activity assay at the concentration of 10-80 mg/ml and Aspirin as reference standard. The experiments were conducted in triplicate. Data was subjected analysis of variance (ANOVA) using SPSS, while values were presented as mean+_standard deviation. Significance was set at p<0.05. according to the results, EOHSS contain bioactive compounds including D-limonene (23.45 ug/ml), ferulic acid (26.27) ug/ml), vanillic acid (15.00 ug/ml), tyrosol (14.66 ppm), naringenin (19.24 ug/ml), epicathechin (9.25 ug/ml), ellagic acid (7.28 ug/ml) and beta pinene (8.04 ug/ml). Appreciable levels of phenols and flavonoids were also detected. The antilipoxygenase activity assay result showed that the highest inflammatory activity of the EOHSS was detected at 80 mg/ml (42.99%) compared to the standard (79.18%). A dose-dependent increase in percentage inhibition was observed as the concentration increased from 20-80 mg/ml of EOHSS. The relatively high phenol and flavonoid contents of EOHSS in this study strongly conti ibute to its anti-inflammatory activity. Hence, it could be exploited for therapeutic purposes against inflammatory related diseases.