Cyanogenic Potential And Partial Purification Of P-Glucosidase From A Cassava (Manihot Esculenta Crantz) Cultivar (ESTRO 0505).:- Nwankwo, Ahamefula A

ABSTRACT The cyanogenic potential and partial purification of B-glucosidasc was carried out on a and biochemical methods. The distribution of cyanogenic glycosides (CNGs) in the leaves, pulp and peel gave cyanide level in parts per million (ppm) 100.188, 168.300 and 45.67, respectively for wet preparation, and 315.216, 18.216 and 22.572, respectively for dry preparation. The carotenoid content ofthe fresh pulp was found to be 4.5mg/g. partial purification using the ammonium sulphate precipitation method yielded an enzyme of activity 5.6units/ml and specific activity of 0.96units/mg protein. The total protein measured from the solution ofthe partially purified enzyme was 5.81mg/ml. ix Key words: Cassava, linamarase, B-glucosidasc, cyanide yield, cyanogenic glycosides, cyanogenic potential, ammonium sulfate, partial purification.