Antimicrobial Activity And Time Kill Kinetics Of Andrographispaniculata Against Some Food Borne Pathogens:- Achigbu, Blessing C

ABSTRACT he plant Andrographicspaniculata has a high antimicrobial activity against selective food borne athogens such as Staphylococcus sp, Escherichia coli, Salmonella sp, Bacillus sp, Shigella and 'seudomonas sp. The extracts ofthe plant were used to conduct a time kill kinetics on the elective food borne pathogens. 'he present study was conducted to investigate the antimicrobial effect of Indrographispaniculata against selective food pathogens. The plant parts of A. panicidata such is leaf, stem and root were studied for its antimicrobial activity. Different solvents were used to :xtract the active components from the plant parts. The antimicrobial activity was studied against elective food pathogens viz., Staphylococcus sp., Escherichia coli, Salmonella sp., Bacillus sp, Shigella and Pseudomonas sp. Among the different solvents the water extract caused inhibitions vith diameters in the range of 10.33mm to 12.33mm. Staphylococcus aureus ,Bacillus, and 'Shigella were all inhibited to the same level (10mm) by the water extract while E-coli, pseudomonas and Salmonella were inhibited with diameters of 11.33mm, 11.67mm, and 12.33mm respectively. The antimicrobial activity of the ethanol extract of A. Paniculata was in the range of 15.33mm (Staphylococcus') to 20.33mm (Pseudomonas), result of minimum inhibitory concentration ofthe plant extract against the food borne pathogens. The water extract had minimum concentration that caused inhibition in the range of lOOmg/ml while that of the ethanol extract was between 50mg/ml and lOOmg/ml. The least concentration of water extract to effect inhibition was 150mg/ml for each of the pathogens except with Salmonella that was inhibited at a minimum concentration of lOOmg/ml ofthe extract