Antibiotic Susceptibility Pattern Of Clinical Isolates Of Staphylococcus Aureus Against Three Groups Of Antibiotics In Umuahia

ABSTRACT

Infections caused by s.aureus is of public health concern due to the easy acquisition of resistance genes. In this research study, the antibiotic sensitivity pattern of clinical isolates of S. aureus in vitro was studied against 3 groups of antibiotics namely quinolones, cephalosporins and aminoglycosides, Susceptibility and resistance were compared and analysed using the disc diffusion method with forty clinical isolates of S.aureus from different specimens ranging from urine to wound and others from hospitals in umuahia. Quinolones were observed to have the greatest antibiotic activity on S. aureus in vitro. S.aureus was found to be most sensitive to pefloxacin (85%) followed by ciprofloxacin (80%). While cephalosporins showed 65% sensitivity, cefuroxin was observed to show 60%. The Aminoglycosides had reduced sensitivity than the other antibiotics used. 

TABLE OF CONTENT

Title page ﾿ i

Certification ﾿ ii

Declaration ﾿ iii

Dedication ﾿ iv

Acknowledgement ﾿ v

Table of Content ﾿ vi ﾿

List of Table ﾿ ix

Abstract ﾿ x

CHAPTER ONE: INTRODUCTION

1.1 ﾿ BACKGROUND ﾿ 1

1.2 IMPORTANCE OF RESEARCH ﾿ 3

1.3. THE AIM AND OBJECTIVE OF RESEARCH ﾿ 4

1.4 OBJECTIVE ﾿ 4

CHAPTER TWO: LITERATURE REVIEW

2.0. THE STAPHYLOCOCCI ﾿ 6

2.1. IMPORTANT PROTERTIES OF STAPHYLOCOCCI ﾿ 6

 2.2. STAPHYLOCOCCUS AUREUS ﾿ 7

2.3. ROLE IN DISEASE ﾿ 8 ﾿

2.4. SKIN INFECTIONS ﾿ 8

2.5. BACTEREMIA. ﾿ 9

 2.6. ANIMAL INFECTIONS ﾿ 9

2.7. VIRULENCE FACTORS ﾿ 10

2.8. STAPHYLOCOCCAL PIGMENTS ﾿ 12

2.9. CLASSICAL DIAGNOSIS ﾿ 13

2.10. TREATMENT AND ANTIBIOTIC RESISTANCE ﾿ 13

2.11. MECHANISM OF RESISTANCE ﾿ 15

2.12. CARRIAGE OF STAPHYLOCOCCUS AUREUS ﾿ 18

2.13. INFECTION CONTROL ﾿ 18

CHAPTER THREE: MATERIALS AND METHOD

3.1. SAMPLES ﾿ 20

3.2. DISC DIFFUSION METHOD ﾿ 20

CHAPTER FOUR: RESULT PRESENTATION

4.1 ﾿ Results ﾿ 21

CHAPTER FIVE

5.1 DISCUSSION ﾿ 27

5.2 CONCLUSION ﾿ 30

5.3 RECOMMENDATION ﾿ 30

Reference

Appendix