Anti-Diabetic Screening Potential Of Methanol Extracts From Alchornea Laxiflora And Dialium Guineense Against Carbohydrate Hydrolyzing Enzymes Andpancreatic Lipase:- Abonyi, Ukamaka S.

ABSTRACT

 Diabetes mellitus is a metabolic disorder of glucose metabolism. The management of blood glucose level is the hallmark in the treatment ofthis disease. This may be achieved through the use of oral hypoglycemic drugs such as sulfonylureas, metformin, insulin secretagogues, and alpha amylase, alpha glucosidase and pancreatic lipase inhibitors. The purpose of the present study was to investigate • Anti-diabetic Screening potential of methanol extract from Alchornea laxiflora and Dialium guineense against carbohydrate hydrolyzing enzymes such as alpha amylase, alpha glucosidase and pancreatic lipase. This was performed using porcine pancreatic alpha amylase from Aspergillus oryzae, alpha glucosidase from Saccharomyces cerevisiae and pancreatic lipase. Highest concentration of methanol extract ofA. laxiflora and extract of D.guineense possesses the highest percentage inhibitory activity against amylase (A.laxiflora Img/ml (60.87%± 0.724) , D.guineense Img/ml (77.34% ±11.33), alpha glucosidase {A.laxiflora 0.2mg/ml (78.44% ± 0.29), D.guineense 0.2mg/ml (66.55%±0.34)and pancreatic lipase (A.laxiflora Img/ml (52.33%± 1.64), D.guineense Img/ml (54.73%±5.66)comparable to the standard drugs, acarbose (80.00% ±0.73) and orlistat (60.03±1.35). It can be concluded that methanol extracts of A.laxiflora . and D.guineense exhibited a potent inhibitory activity on the three enzymes studied.