Vitro Effects of Ethanolic Extracts of Pterocarpus santalinoides Leaves on the Isolated Uterine I issues of Non -Gravid Albino Wistar Rat:- Iheonunekwu, Goodness Chigozirim
ABSTRACT
The aim of this study was to determine the
effect of the Ethanolic Extracts of Pterocarpus Santalinoides leaves (EEPSL) on
isolated uterine smooth muscle of non-gravid rats and to deduceits possible
mechanism(s) of action. The extract of Pterocarpus santalinoides leaves
mimicked Oxytocin in producing a concentration dependent uterine contraction
and the maximum response which was significant (P < 0.05) was observed at
the least concentration (14.25mm. 0.33mg/ml). Increasing the concentration of
the extract (1.33mg/ml) did not increase the response of the extract on the
isolated uterine smooth muscle but rather, recorded the least response effect
(with amplitude of 8.50mm). Similar to Oxytocin, the percentage activity was
highest and significant (P < 0.05) at the least concentration (271.25%,
0.33mg/ml) though not significant (P > 0.05) with the percentage activity
recorded at the double concentration 0.66mg/ml and 1.33mg/ml (243.75% and
197.90%) respectively. Further investigation to deduce the possible mechanism
of action of the extract showed that independent introduction of a calcium
channel blocker Verapamil (0.0067mg/ml), and a selective B adrenergic blocker
Salbutamol (0.0067mg/ml) respectively, completely abolished the EEPSL
(0.33mg/ml) induced uterine force thereby showing involvement of mobilization
of extracellular calcium and stimulation of-adrenegic receptors in the
mechanism of action. The findings of this study suggests that EEPSL may possess
potent uterotonic principle(s) hence the traditional use of the plant for
flushing unwanted uterine contents post-partum. Further studies are hereby
recommended to isolate and purify and characterize the active ingredient
responsible for this contractile effect.
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MICHAEL, U (2024). Vitro Effects of Ethanolic Extracts of Pterocarpus santalinoides Leaves on the Isolated Uterine I issues of Non -Gravid Albino Wistar Rat:- Iheonunekwu, Goodness Chigozirim. Repository.mouau.edu.ng: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/vitro-effects-of-ethanolic-extracts-of-pterocarpus-santalinoides-leaves-on-the-isolated-uterine-i-issues-of-non-gravid-albino-wistar-rat-iheonunekwu-goodness-chigozirim-7-2
UNIVERSITY, MICHAEL. "Vitro Effects of Ethanolic Extracts of Pterocarpus santalinoides Leaves on the Isolated Uterine I issues of Non -Gravid Albino Wistar Rat:- Iheonunekwu, Goodness Chigozirim" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 26 Feb. 2024, https://repository.mouau.edu.ng/work/view/vitro-effects-of-ethanolic-extracts-of-pterocarpus-santalinoides-leaves-on-the-isolated-uterine-i-issues-of-non-gravid-albino-wistar-rat-iheonunekwu-goodness-chigozirim-7-2. Accessed 24 Nov. 2024.
UNIVERSITY, MICHAEL. "Vitro Effects of Ethanolic Extracts of Pterocarpus santalinoides Leaves on the Isolated Uterine I issues of Non -Gravid Albino Wistar Rat:- Iheonunekwu, Goodness Chigozirim". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 26 Feb. 2024. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/vitro-effects-of-ethanolic-extracts-of-pterocarpus-santalinoides-leaves-on-the-isolated-uterine-i-issues-of-non-gravid-albino-wistar-rat-iheonunekwu-goodness-chigozirim-7-2 >.
UNIVERSITY, MICHAEL. "Vitro Effects of Ethanolic Extracts of Pterocarpus santalinoides Leaves on the Isolated Uterine I issues of Non -Gravid Albino Wistar Rat:- Iheonunekwu, Goodness Chigozirim" Repository.mouau.edu.ng (2024). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/vitro-effects-of-ethanolic-extracts-of-pterocarpus-santalinoides-leaves-on-the-isolated-uterine-i-issues-of-non-gravid-albino-wistar-rat-iheonunekwu-goodness-chigozirim-7-2