ABSTRACT

 The effect of various concentrations of aqueous mixture of substanaces (salt and Alcohol): 60g/L/kg body weight (high close) and 10g/L/kg body weight (low dose) for salt and alcohol ( ic:total ethanol 98%) 6ml in 940m1 of distilled water per kg body weight (high dose) and 3m1 in 940ni1 1)/W/kg body weight (Low dose) on sonic biochemical indices of rat liver function: Alanine and aspartate aminotransferases, and Alkaline phosphatase. Blood urea nitrogen (Bun), total protein concentration and electrolytes: sodium, potassium, chloride and bicabonate were investigated on hypertensive albino wister strained rats. Five groups were considered in the cause of this study ranging from group I through V. group I (GPI) hypertensive on high salt dose. Group 11 (UP1I) is the high salt group control subjected to low salt supplement. Group III (GPIJI) is the hypertensive group subjected of high alcohol concentration. Group IV (GP IV) S tile high alcohol group control on low alcohol supplement. Group V is the general control group fed on normal feed and distilled water Only. Each of the five groups were made up or six albino rats each. Administration of high closes of substances containing salt and alcohol were given to group I and 111 and a low dose of salt to group II produced mean weight loss of -47.73 ± 1.98, and -49.07 ± 3.09 and -21.95 ± 1.00 respectively. which group IV and group V (Control) gained mean weight of 30.57 ± 1.80, and 26.45 ± 2.88g. Blood urea Nitrogen level was elevated at high and low doses of salt concentration as seen in GPI (120.50 ± 3.64iu/L) and Gp11 (81.33 ± i.49iu/L) but remained unaffected at low and high concentration of alcohol in GPIII (43.25 ± 1 .09iu/L) and OP J\/ (25.33 ± 0.94), while GE\ remained normal. Serum protein decreased in OPI (2.40 ± 0.08iu/L) and GP 11(4.42 ± 0.23 iu/L), Groups 111 (high alcohol dose) and V were found to be unaffected having a macan of 7.45 ± 0.34iu/L and 7.50 ± 0.00 and there was a significant elevation i1 OP IV (Low alcohol dose) all having their elevation or decrease being concentration dependent. Serum sodium, polassium and chloride levels in the blood was found to be elevated in all groups administered substances apart & omn group V (control) that maintained normal values. 96.67 ± 0.75ui/L, 4.07 ± 0.09iulL and 138.50 ± 0.76ui/L were for chloride, potassium and sodium respectively. Serum bicarbonates decreased in all the groups apart from OP V maintaining a normal value of 25.00 ± 0.58. Liver enzyme activities of Alkaline Phosnliatne. Alanine Aminotransferase and Aspartate Aminotransferase elevated in groups I and Iii only being groups led on high concentrations of Salt and alcohol. . Groups II, IV and V were all found to have normal ranges in 1\LP, ALT, AS'I' enzymes. Hence, their level of elevation or decrease depends on the administered concentration. Mean relative liver weight for the groups decreased from 0.0501 ± 0.00224 OP 111, 0.0493 ± 0.00505 GP I, 0.0402 ± 0.058 OP II, 0.392 ± 0.00290 GP V to 0.034 1 ± 0.00114 OP IV. Mean relative kidney weight followed in the same orders as that of the mean relative liver weight for the groups Ito V. This indicates that the substances of salt and alcohol in high closes increases the weight of both liver and kidney when compared with the control: hence reflecting the level of damages caused by the administration of these substances on the liver and kidney of hypertensive patients.