Partial Purification And Some Properties Of B-Glucosidase From Rubber (Hevea Brasiliensis) Seed.
ABSTRACT
The Hydrolytic Activity of Endogenous
B-Glucosidsc Of Tevea Brasiliensis Towards The Cyanogenic Glycosides Of Rubber
Seeds Was Investigated, Followed Dy The Study Of The Partial Purification And
Some Kinetic Properties Of The Enzyme. Crushing Of The Rubber Seeds And
Allowing The Interaction Of The Endogcnous B -Glucosidase And The Cyanogenic
Glycosides Content Of The Seeds For A Period Of About 60 Mili Resulted In About
90 95% Hydrolysis Of The Evanogenic Glycosides By The Endogenous Enzyme.
((Ni-I4)2 So4precipitation Of Lire Enzyme Followed By Dialysis Gave A Artiahly
Purified Enzyme Of' Activity 6.5 Units/Mi And Specific Activity Of 6.L9units/Mg
Protein. The Total Protein Measured From The Solution Of The Crude And
Partially Purified Enzyme Was 8.35mg/Mi Arid 1.05mg/Mi. Mathematical Treatment
Of The Data From The Degradation Of' Linamarin By The Partially Purified Enzyme
Generated Cn- Values That Were Used To Construct A Lineweaver- Burk Plot Which
Gave Km And Vmax Values Of 38.46mm And 6.9x10 Pmolcn7min/Ml Enzyme.
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ANYANWU, O (2021). Partial Purification And Some Properties Of B-Glucosidase From Rubber (Hevea Brasiliensis) Seed. . Repository.mouau.edu.ng: Retrieved Dec 21, 2024, from https://repository.mouau.edu.ng/work/view/partial-purification-and-some-properties-of-b-glucosidase-from-rubber-hevea-brasiliensis-seed-7-2
ONYEMA, ANYANWU. "Partial Purification And Some Properties Of B-Glucosidase From Rubber (Hevea Brasiliensis) Seed. " Repository.mouau.edu.ng. Repository.mouau.edu.ng, 30 Jun. 2021, https://repository.mouau.edu.ng/work/view/partial-purification-and-some-properties-of-b-glucosidase-from-rubber-hevea-brasiliensis-seed-7-2. Accessed 21 Dec. 2024.
ONYEMA, ANYANWU. "Partial Purification And Some Properties Of B-Glucosidase From Rubber (Hevea Brasiliensis) Seed. ". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 30 Jun. 2021. Web. 21 Dec. 2024. < https://repository.mouau.edu.ng/work/view/partial-purification-and-some-properties-of-b-glucosidase-from-rubber-hevea-brasiliensis-seed-7-2 >.
ONYEMA, ANYANWU. "Partial Purification And Some Properties Of B-Glucosidase From Rubber (Hevea Brasiliensis) Seed. " Repository.mouau.edu.ng (2021). Accessed 21 Dec. 2024. https://repository.mouau.edu.ng/work/view/partial-purification-and-some-properties-of-b-glucosidase-from-rubber-hevea-brasiliensis-seed-7-2