ABSTRACT

A total of Ten (10) Samples were collected from  barbing salons in Umuahia Metropolis with the aid of sterile swab stick and transported to laboratory for microbial analysis. The biochemical characterization of  isolates revealed the genera of  Pseudomonas sp, Bacillus sp, E.coli, Micrococcus luteus, Proteus sp and Serratia marcesens  and the fungal characterization revealed the presence of Aspergillus niger, Rhodotorula sp, Fusarium oxyporum and Aspergillus flavus. The percentage occurrence of isolates from user hair brushes and clippers in barbing salons samples showed that Pseudomonas sp had the highest occurrence (25%) followed by E.coli 18.75%, Micrococcus luteus (18.75%) , Proteus sp (12.5%), while Serratia marcesens (6.25%) while Bacillus sp (6.25%) had the least occurrence. The percentage occurrence of fungal isolates from user hair brushes and clippers in barbing salons samples showed that Aspergillus niger (38.5%) had the highest percentage occurrence followed by Rhodotorula sp (30.8%) while Fusarium oxysporum (15.4%)  and Aspergillus flavus (15.4%) showed the lowest occurrence from user hair brushes and clippers in barbing salons samples. Antibiotics sensitivity test of the isolated bacteria revealed that all the six recorded (100%) sensitivity on Ciprofloxacin and Gentamycin while Proteus sp, Pseudomonas aeruginosa and E.coli were all resistant to Levofloxacin and Rifampicin respectively.

TABLE OF CONTENTS

Title Page i

Certification ii

Dedication iii

Acknowledgements iv

Table of Contents v

Lists of Tables vii

Abstract viii

CHAPTER ONE

1.0 Introduction 1

1.1 Aim and Objectives  3

CHAPTER TWO 

2.0  Literature Review 4

2.1 Folliculitis 9

2.2 Tinea    10

CHAPTER THREE

3.0 Materials and Methods  14

3.1 Sample Collection 14

3.2 Media Used  14

3.3 Sterilization  15

3.5 Determination of Bacterial Isolation  15

3.5.1 Grams Staining  16

3.6 Biochemical Characteristics of The Isolates  16

3.6.2 Indole Test  17

3.6.3 Citrate Utilization 17

3.6.4 Sugar Fermentation 17

3.6.5 Oxidase Test 18

3.6.6 Coagulase Test 18

3.6.7 Urease Test 18

3.6.8 Methyl Red –Voges Proskauer (MRVP) Test 19

3.7 Characterization And Identification of The Fungal isolates 19

3.7.1 Lactophenol Cotton Blue Staining 20

3.8 Antibiotic Susceptibility Testing  20

CHAPTER FOUR

4.0  Results 21

CHAPTER FIVE

5.0       Discussion, Conclusion and Recommendation 30

5.1 Discussion 30

5.2 Conclusion 32

5.3 Recommendation 32

References