Isolation Partial Purification And Some Properties Of Crude Gut Enzymes From The African Giant Snail (Ac1uitina Ac/Iathui) Used For Yeast Protaoplast Production
ABSTRACT
The
crude gut enzyme of the African giant snail was partially purified by extraction
with acetate buffer, fractional precipitation with ammonium sulphate and
acetone followed by dialysis and heat treatment. The protein content of the crude.
enzyme extract was 1 .05mg/mi, while that of ammonium suii5hate and acetone
fractions were 0:75mg/mi and 0.65mg/ml respectively. The partially purified
enzyme fractions had enzyme activity of 4.70U/mi for ammonium suiphite and 3
.80U/ml for acetone while that of the crude extract was 1 .60U/ml. The
temperature range for the enzyme activity of both the crude and the jartially
purified fractions were 25°-60°C, while the pH range was 5.0- 6.0.The production
of yeast protoplasts was achieved using both the crude and the partially
purified fractions. There was 238% increase in protoplasts produced by the
acetone fraction and 294% increase by the ammonium sulphate fraction when
compared with the number of protoplasts produced by the crude extract. The
statistical analysis showed significant differences (P<0.05) of protoplasts production
from the various purification methods. Protoplasts production increased with
higher concentrations and higher incubation period. Optimum results of 3.95 x
106 protoplasts/mi for Saccharomyces cerevisiae and 115 x 1 6 protopiasts/tnl
for Pichia pastoris were obtained when equal volume of • stabilized yeast cells
and ammonium sulphate fraction of the partially purified enzyme were mixed;
incubated at 30°C for 120minutes with 1.OM sorbitol as osmotic stabilizer and
at a pH of 6.0. The results obtained from this study can 'provide key factors
for optimum yeast protoplasts production using the gut enzymes of Achatina
achatina and offer a good means of commercial utilization of this otherwise
wasted enzymes.
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MBAJIUKA, S (2021). Isolation Partial Purification And Some Properties Of Crude Gut Enzymes From The African Giant Snail (Ac1uitina Ac/Iathui) Used For Yeast Protaoplast Production . Repository.mouau.edu.ng: Retrieved Nov 23, 2024, from https://repository.mouau.edu.ng/work/view/isolation-partial-purification-and-some-properties-of-crude-gut-enzymes-from-the-african-giant-snail-ac1uitina-aciathui-used-for-yeast-protaoplast-production-7-2
STANLEY, MBAJIUKA. "Isolation Partial Purification And Some Properties Of Crude Gut Enzymes From The African Giant Snail (Ac1uitina Ac/Iathui) Used For Yeast Protaoplast Production " Repository.mouau.edu.ng. Repository.mouau.edu.ng, 02 Jun. 2021, https://repository.mouau.edu.ng/work/view/isolation-partial-purification-and-some-properties-of-crude-gut-enzymes-from-the-african-giant-snail-ac1uitina-aciathui-used-for-yeast-protaoplast-production-7-2. Accessed 23 Nov. 2024.
STANLEY, MBAJIUKA. "Isolation Partial Purification And Some Properties Of Crude Gut Enzymes From The African Giant Snail (Ac1uitina Ac/Iathui) Used For Yeast Protaoplast Production ". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 02 Jun. 2021. Web. 23 Nov. 2024. < https://repository.mouau.edu.ng/work/view/isolation-partial-purification-and-some-properties-of-crude-gut-enzymes-from-the-african-giant-snail-ac1uitina-aciathui-used-for-yeast-protaoplast-production-7-2 >.
STANLEY, MBAJIUKA. "Isolation Partial Purification And Some Properties Of Crude Gut Enzymes From The African Giant Snail (Ac1uitina Ac/Iathui) Used For Yeast Protaoplast Production " Repository.mouau.edu.ng (2021). Accessed 23 Nov. 2024. https://repository.mouau.edu.ng/work/view/isolation-partial-purification-and-some-properties-of-crude-gut-enzymes-from-the-african-giant-snail-ac1uitina-aciathui-used-for-yeast-protaoplast-production-7-2