Isolation And Identification Of Streptomyces Species With Antifungal Activity Against Fungal Phytopathogens Causing Pawpaw Rot
ABSTRACT
The present study isolated and identified Streptomyces species from soil samples having antifungal activity against selected fungal phytopathogens (Aspergillus niger, Rhizopus stolonifer, Fusarium solani, Aspergillus flavus). Soil samples were collected from different locations of Michael Okpara University of Agriculture, Umudike (MOUAU). The 10 soil samples were cultured by spread plate inoculation method on Nutrient Agar, Sabouraud Dextrose Agar and Tryptone Soya Agar media. Totally 13 Streptomyces species were isolated and tested for antifungal activity by agar well diffusion method against the fungal phytopathogenic microorganisms. Isolate IS12, IS51, IS72, and IS91 were moderately to highly active, while IS51 exhibited the highest antifungal activity against Aspergillus niger (19mm) and Aspergillus flavus (18mm) and IS72 showed the highest activity against Rhizopus stolonifer (19mm) and Fusarium solani (16mm). The zone of inhibition for crude extracts were also determined by agar well diffusion method. These isolates had antifungal activity and could be used in the development of novel antibiotics for agricultural purposes or employed as biocontrol agents to controlling plant (pawpaw) fungal diseases. Soil samples could be an interesting source to explore for antifungal secondary metabolites and there is not any scientific report on the isolation of Streptomyces species producing antifungal metabolites from the areas of study.diffusion method. These isolates had antifungal activity and could be used in the development of novel antibiotics for agricultural purposes or employed as biocontrol agents to controlling plant (pawpaw) fungal diseases. Soil samples could be an interesting source to explore for antifungal secondary metabolites and there is not any scientific report on the isolation of Streptomyces species producing antifungal metabolites from the areas of study.
CHAPTER ONE
1.0Introductio 1
1.1 aims and objectives- - - - - - - - - 4
CHAPTER TWO
2.0 Literature of Review- - - - - - - - -
CHAPTER THREE
MATERIALS AND METHODS
CHAPTER FOUR
4.0 Results
CHAPTER FIVE
5.0 Discussion
5.1Conclusion
5.2 Recommendation
Appendix
0.0
ODO, O (2020). Isolation And Identification Of Streptomyces Species With Antifungal Activity Against Fungal Phytopathogens Causing Pawpaw Rot. Repository.mouau.edu.ng: Retrieved May 13, 2024, from https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-streptomyces-species-with-antifungal-activity-against-fungal-phytopathogens-causing-pawpaw-rot
ODO, ODO. "Isolation And Identification Of Streptomyces Species With Antifungal Activity Against Fungal Phytopathogens Causing Pawpaw Rot" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 11 May. 2020, https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-streptomyces-species-with-antifungal-activity-against-fungal-phytopathogens-causing-pawpaw-rot. Accessed 13 May. 2024.
ODO, ODO. "Isolation And Identification Of Streptomyces Species With Antifungal Activity Against Fungal Phytopathogens Causing Pawpaw Rot". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 11 May. 2020. Web. 13 May. 2024. < https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-streptomyces-species-with-antifungal-activity-against-fungal-phytopathogens-causing-pawpaw-rot >.
ODO, ODO. "Isolation And Identification Of Streptomyces Species With Antifungal Activity Against Fungal Phytopathogens Causing Pawpaw Rot" Repository.mouau.edu.ng (2020). Accessed 13 May. 2024. https://repository.mouau.edu.ng/work/view/isolation-and-identification-of-streptomyces-species-with-antifungal-activity-against-fungal-phytopathogens-causing-pawpaw-rot