Abstract
This study
was aimed at investigating microorganisms associated with biogas productipn
using" cassava peel as substrates. Cassava peels obtained after peeling
cassava roots were anaerobically digested using a 5 litre capacity fermentor.
The peels were blended and mixed with water, in the ratio of 1:2. Standard
microbiological methods and anaerobic biodigester were used to screen the
isolates and the wastes substrate for biogas production. The results ofthe
total heterotrophic and anaerobic microbial counts during the digestion period
showed a steady variation in the anaerobic bacteria and fungal counts as
fermentation progressed. The load obtained for the total heterotrophic
bacteria'and anaerobic bacteria were within 2.9xl04cfu/ml to 9.4xl04cfu/ml and
1.8xl03cfu/ml to 6.9x103cfu/ml respectively. Similarly the fungal count
obtained through the digestion period ranged from l.lxlOcfu/ml to 3.9xl0cfu/ml.
The bacterial isolates were identified as Pseudomonas sp, Escherichia coli,
Bacillus sp, Clostridium sp, and Methanococcus sp, while fungi isolated were
identified as Saccharomyces cerevicae, Aspergillus sp and . Penicillium sp. The
cumulative flammable biogas yield of the cassava peels was 13.7dm3/total . mass
of slurry. Analysis revealed that the temperature of raw substrates ranged
between 26°C . and 30°C while the pH varied between 4.04 and 6.86 during
digestion
NNABUGWU (2026). Isolation And Characterization Of Microorganisms Involved In Biogas Production Using Cassava Peels:- Nnabugwu, Winner C. Repository.mouau.edu.ng: Retrieved Mar 13, 2026, from https://repository.mouau.edu.ng/work/view/isolation-and-characterization-of-microorganisms-involved-in-biogas-production-using-cassava-peels-nnabugwu-winner-c-7-2
NNABUGWU. "Isolation And Characterization Of Microorganisms Involved In Biogas Production Using Cassava Peels:- Nnabugwu, Winner C" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 13 Mar. 2026, https://repository.mouau.edu.ng/work/view/isolation-and-characterization-of-microorganisms-involved-in-biogas-production-using-cassava-peels-nnabugwu-winner-c-7-2. Accessed 13 Mar. 2026.
NNABUGWU. "Isolation And Characterization Of Microorganisms Involved In Biogas Production Using Cassava Peels:- Nnabugwu, Winner C". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 13 Mar. 2026. Web. 13 Mar. 2026. < https://repository.mouau.edu.ng/work/view/isolation-and-characterization-of-microorganisms-involved-in-biogas-production-using-cassava-peels-nnabugwu-winner-c-7-2 >.
NNABUGWU. "Isolation And Characterization Of Microorganisms Involved In Biogas Production Using Cassava Peels:- Nnabugwu, Winner C" Repository.mouau.edu.ng (2026). Accessed 13 Mar. 2026. https://repository.mouau.edu.ng/work/view/isolation-and-characterization-of-microorganisms-involved-in-biogas-production-using-cassava-peels-nnabugwu-winner-c-7-2