Isolation And Characterization Of Bioactive Natural Products In Justicia spicigera (Acanthaceae)

OFFIONG NELSON | 23 pages (44165 words) | Theses
Chemistry | Co Authors: OHENHEN

ABSTRACT

The Phytochemical evaluation of the secondary metabolites in Justicia spicigera using n-hexane, methanol, chloroform and ethanol extracts revealed the bioavailability of bioactive compounds comprising alkaloids (3.048 - 8.265 mg/100 g), tannins (0.080-0.254 mg/100 g), phenols (0.014 - 0.029 mg/100 g), saponins (0.564-0.744 mg/100 g), flavonoids (0.780-9.820 mg/100 g) and steroids (0.667-1.277 mg/100 g). Minerals content revealed the presence of magnesium (1.094 -1.277 mg/100 g), calcium (3.707-4.509 mg/100 g), potassium (1.2-1.3 mg/100 g), sodium (0.325 - 0.55 mg/100 g), phosphorous (0.689-0.708 mg/100 g)., manganese (5.80-7.98 mg/100 g) and the Trace element analysis revealed - zinc (11.13-15.10 mg/100 g), copper (4.88-4.98 mg/100 g), iron (51.30-53.08 mg/100 g) and lead (1.60-2.46 mg/100 g). The vitamins content was ascorbic acid (92.40-57.20 mg/100 g), β-carotenoid (312.50-474.54 mg/100 g), riboflavin (2.63-4.13 mg/100 g), thiamin (0.38-0.75 mg/100 g) and niacin (0.84-1.41 mg/100 g). Extracts of Justicia spicigera prepared with solvents of different polarities (n-Hexane, Chloroform, Methanol, and Ethanol) were used to analyze the anti-bacterial properties using the test microorganisms Streptococcus pneumonia, Salmonella typhi, Escherichia coli and Aspergilus niger with the positive control as Ciprofloxacin. Minimum inhibitory concentration (MIC) was attained at 125-250 mg/ml for E.coli, S. typhi, and S. pneumonia and (MIC) was attained at 250-500 mg/ml for Aspergilus niger. Chemical investigation of the leaves of a herbaceous medicinal plant Justicia spicigera resulted in the isolation of two natural products – Compound S18 and Compound S21 from the crude methanol extract of the leaves. Isolation of Compound S18 and Compound S21 was carried out by Column Chromatography using silica gel as the adsorbent. The composition of the crude mixture was examined using thin layer chromatography. The combined Infra-red, Proton and DEPT Nuclear Magnetic Resonances, Mass Spectroscopy, 1H-1H COSY and 1H-13C-HMBC were used as spectral techniques to propose the structures of the compounds. The High Resolution Electro-Spray Impact Mass Spectroscopy analysis of Compound S18 showed a molecular ion (M+) peak at m/z C35H58O8 and Compound S21 of molecular ion (M+) peak at m/z C31H47O16. The characterization of the isolated natural products from Justicia spicigera revealed Compound S18 is a triterpenoidal glycoside and Compound S21 is confirmed to be a δ-lactone glycoside. The presence of bioactive natural products may be a contributor to the antimicrobial, antioxidant, anti-inflammatory, liver protector, analgesic, anti-cancer, anti-diabetic and antacid properties exhibited by Justicia spicigera. Justicia spicigera offers a wide scope for pharmaceutical industries to use in drug formulations.

 

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APA

OFFIONG, N (2022). Isolation And Characterization Of Bioactive Natural Products In Justicia spicigera (Acanthaceae). Repository.mouau.edu.ng: Retrieved Jan 26, 2023, from https://repository.mouau.edu.ng/work/view/isolation-and-characterization-of-bioactive-natural-products-in-justicia-spicigera-acanthaceae-7-2

MLA 8th

NELSON, OFFIONG. "Isolation And Characterization Of Bioactive Natural Products In Justicia spicigera (Acanthaceae)" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 26 Oct. 2022, https://repository.mouau.edu.ng/work/view/isolation-and-characterization-of-bioactive-natural-products-in-justicia-spicigera-acanthaceae-7-2. Accessed 26 Jan. 2023.

MLA7

NELSON, OFFIONG. "Isolation And Characterization Of Bioactive Natural Products In Justicia spicigera (Acanthaceae)". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 26 Oct. 2022. Web. 26 Jan. 2023. < https://repository.mouau.edu.ng/work/view/isolation-and-characterization-of-bioactive-natural-products-in-justicia-spicigera-acanthaceae-7-2 >.

Chicago

NELSON, OFFIONG. "Isolation And Characterization Of Bioactive Natural Products In Justicia spicigera (Acanthaceae)" Repository.mouau.edu.ng (2022). Accessed 26 Jan. 2023. https://repository.mouau.edu.ng/work/view/isolation-and-characterization-of-bioactive-natural-products-in-justicia-spicigera-acanthaceae-7-2

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