ABSTRACT

The study was aimed at assessing asymptomatic bacteriuria among female students of Michael Okpara University of Agriculture, Umudike, Abia State. Randomly sampled clean catch midstream urine specimens were collected from 110 apparently healthy asymptomatic undergraduate female students of MOUAU between ages of 16 to 30 years. The urine specimen was cultured on Cysteine Lactose Electrolyte Deficient Agar (CLED), which is the gold standard for detecting asymptomatic bacteriuria and incubated overnight at 370C. This reveals that out of the 110 urine specimen culture, forty two (42) urine specimen showed Scanty, whitish circular growth on the media, two (2) urine specimen showed Yellowish circular growth, fourteen (14) urine specimen showed Large circular, yellowish cluster growth, twelve (12) urine specimen showed Moderate growth circular, yellowish, two (2) urine specimen showed Whitish, circular, crossed straight and forty six (46) urine specimen showed no sign of growth on the culture media.The results showed that the total prevalence rate of bacteriuria was Coagulase Negative Staphylocccus 21(32.82%) was most common uropathogen isolated followed by Escherichia coli 5(7.81%), Staphylococcus aurues 15(23.43%), Proteus species 2(3.12%), Enterococcus spp 10(15.62%), Group B Streptococcus 2(3.12%), Citrobacter spp 5(7.81%), and Klebsiella spp 3(4.68%) respectively while Pseudomonas spp 1(1.56%) had the lowest. Coagulase Negative Staphylococcus was the most common bacteria for asymptomatic bacteriuria in female university undergraduates in this study. The incidence of the bacteriuria in a healthy population is of significant public health concern as they may be incubating pathogenic microorganism which may cause disease they may be incubating pathogenic Microorganism which may cause disease when they interact with the opposite sex. Thus, education and awareness on the need for proper personal hygiene should be encouraged.

Title Page   i

Certification iii

Dedication iv

Acknowledgement v

Table of Contents vi

List of Tables vii

Abstract ix

CHAPTER ONE: INTRODUCTION 1

1.1 Aims and Objectives 2

CHAPTER TWO: LITERATURE REVIEW 4

2.1 Asymptomatic Bacteriuria 4

2.2 Prevalence of Asymptomatic Bacteriuria 5

2.3 The Role of Asymptomatic Bacteriuria Prior to Surgery 6

2.4 The Role of Asymptomatic Bacteriuria in Women Affected by Recurrent 

Urinary Tract Infections 7

2.5 Screening for Asymptomatic Bacteriuria 8

2.6 Asymptomatic Bacteriuria amongst Female Students of Rivers State 

University of Science and Technology, Port Harcourt 9

2.7 Asymptomatic Bacteriuria among Female Students of a Tertiary 

Institution in Southeast Nigeria 10

2.8 Microorganisms Associated with Asymptomatic Bacteriuria 12

CHAPTER THREE: MATERIALS AND METHODS 14

3.1 Study Area 14

3.2 Study Population 14

3.3 Sample Collection 14

3.4 Microbial Analysis 15

3.5 Urine Microscopy 15

3.6 Media Used 15

3.7 Sample Preparation and Isolation of Microorganisms 16

3.7.1 Isolation of Microorganisms 16

CHAPTER FOUR: RESULTS 17

CHPATER FIVE: DISCUSSION, CONCLUSION AND RECOMMENDATION 20

5.1 Discussion 20

5.2 Conclusion 22

5.3 Recommendation 22

References