In Vitro Effects of Ethanolic Extracts of Pterocarpus santalinoides Leaves on the Isolated Uterine Tissues of Non -Gravid Albino Wistar Rats:- Iheonunekwu, Goodness Chigozirim
ABSTRACT
The aim of this study was to
determine the effect of the Ethanolic Extracts of Pterocarpus Santalinoides leaves
(EEPSL) on isolated uterine smooth muscle of non-gravid rats and to deduce its
possible mechanism(s) of action. The extract of Pterocarpus santalinoides
leaves mimicked Oxytocin in producing a concentration dependent uterine
contraction and the maximum response which was significant (P < 0.05) was
observed at the least concentration (14.25mm, 0.33mg/ml). Increasing the
concentration of the extract (1.33mg/ml) did not increase the response of the
extract on the isolated uterine smooth muscle but rather, recorded the least
response effect (with amplitude of 8.50mm). Similar to Oxytocin, the percentage
activity was highest and significant (P < 0.05) at the least concentration
(271.25%, 0.33mg/ml) though not significant (P > 0.05) with the percentage
activity recorded at the double concentration 0.66mg/ml and 1.33mg/ml (243.75%
and 197.90%) respectively. Further investigation to deduce the possible
mechanism of action of the extract showed that independent introduction of a
calcium channel blocker Verapamil (0.0067mg/ml), and a selective B adrenergic
blocker Salbutamol (0.0067mg/ml) respectively, completely abolished the EEPSL
(0.33mg/ml) induced uterine force thereby showing involvement of mobilization
of extracellular calcium and stimulation of-adrenegic receptors in the
mechanism of action. The findings of this study suggests that EEPSL may possess
potent uterotonic principle(s) hence the traditional use of the plant for
flushing unwanted uterine contents post-partum. Further studies are hereby
recommended to isolate and purify and characterize the active ingredient
responsible for this contractile effect.
0.0
GOODNESS, C (2023). In Vitro Effects of Ethanolic Extracts of Pterocarpus santalinoides Leaves on the Isolated Uterine Tissues of Non -Gravid Albino Wistar Rats:- Iheonunekwu, Goodness Chigozirim. Repository.mouau.edu.ng: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/in-vitro-effects-of-ethanolic-extracts-of-pterocarpus-santalinoides-leaves-on-the-isolated-uterine-tissues-of-non-gravid-albino-wistar-rats-iheonunekwu-goodness-chigozirim-7-2
CHIGOZIRIM, GOODNESS. "In Vitro Effects of Ethanolic Extracts of Pterocarpus santalinoides Leaves on the Isolated Uterine Tissues of Non -Gravid Albino Wistar Rats:- Iheonunekwu, Goodness Chigozirim" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 18 Oct. 2023, https://repository.mouau.edu.ng/work/view/in-vitro-effects-of-ethanolic-extracts-of-pterocarpus-santalinoides-leaves-on-the-isolated-uterine-tissues-of-non-gravid-albino-wistar-rats-iheonunekwu-goodness-chigozirim-7-2. Accessed 24 Nov. 2024.
CHIGOZIRIM, GOODNESS. "In Vitro Effects of Ethanolic Extracts of Pterocarpus santalinoides Leaves on the Isolated Uterine Tissues of Non -Gravid Albino Wistar Rats:- Iheonunekwu, Goodness Chigozirim". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 18 Oct. 2023. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/in-vitro-effects-of-ethanolic-extracts-of-pterocarpus-santalinoides-leaves-on-the-isolated-uterine-tissues-of-non-gravid-albino-wistar-rats-iheonunekwu-goodness-chigozirim-7-2 >.
CHIGOZIRIM, GOODNESS. "In Vitro Effects of Ethanolic Extracts of Pterocarpus santalinoides Leaves on the Isolated Uterine Tissues of Non -Gravid Albino Wistar Rats:- Iheonunekwu, Goodness Chigozirim" Repository.mouau.edu.ng (2023). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/in-vitro-effects-of-ethanolic-extracts-of-pterocarpus-santalinoides-leaves-on-the-isolated-uterine-tissues-of-non-gravid-albino-wistar-rats-iheonunekwu-goodness-chigozirim-7-2