ABSTRACT

This study examines the in-vitro assessment of propolis from three different vegetation zones (f Nigeria and determination of its flavonoids and ferulic acid contents. The objectives of this study are to determine the flavonoids and ferulic acid contents of propolis from the three vegetatioa zones and their relationship with microbial activities. Different concentrations of propohs extracts (ethanol and water) were prepared, media for the microbial growth were prepared using nutrients agar for bacteria growth and sasourand agar for fungi growth .O.5m1 of the cultured pathogen were introduced in a petri dish and 1 ml of each propolis extract concentration were added. The bacteria plates were incubated for 24-48hrs while the fungi inoculated plates were inoculated for 2-5 days with daily observation. On establishment of the growth, the plates were examined for the presence of inhibition zone around each embedded disc.The result showed that the flavonoids contents ranged from 8.76 to 10.33mg/lOOg with the derived savanna having the highest flavonoid content.the ferulic acid contents of the propolis samples ranged from 3.32 to 3.71mg/bOg with rainforest having the highest ferulic acids content. the water extracted derived savanna propolis had the highest effect on streptococcus spp at 500mg/ml this may be due to that flavonoids are the main propolis contents that makes it medicinal. The ethanol extracted rainforest vegetation propolis gave the highest effect on Aspergillus spp at 500mg1ntl concentration. In conclusion,the effectiveness of propolis sample extracts increased with increa e in concentration. From this study I recommend that modern beekeeping should be encouraged because propolis comes from beehives.