ABSTRACT
Many disorders today fi ix J. c Many disorders
today are based on the imbalances of the immunological processes. This
necessitates the search for newer and safer immunomodulators. Thus, the objective
of this present study was to explore the immunomodulatory activity of the
methanol leaf extract of Alslonia boonei. The effect of the plant extract On
CD4+ count. C-reactive protein and cytokine (interleukin-10) expression were
measured in Wistar rats. Twelve (12) Wistar rats were used for this studv and
were randomly divided into 3 groups (n=4), after which they were acclimatized
for a period oftwo weeks. The normal control served as group 1 and received
only feed and water ad libitum. Group 2 (standard drug group) received standard
drug (Immunace) while group 3 received the plant extract at 400 mg/kg body
weight. Extraction of the plant was carried out by soaking grinded leaves of A.
boonei in methanol for 72 hours followed by filtration and concentration of
filtrate in a beaker using a w'ater bath. The extract and the standard drug
were then administered to the animals according to their body weight. This
administration lasted for a period of three (3) w eeks after which blood was
collected through cardiac puncture and used for immunological tests for CD4+
count, C-reactive protein and interleukin-10. Results obtained hows that there
was a significant (p<0.05) increase in interleukin-10 for the plant extract
treated group (181.53+4.29) compared to the normal control (168.45+4.95) and
the standard drug group (145.33+4.13).CD4 count shows a non significant
(p<0.05) increase in the plant extract treated group (14.50+1.29) when
compared to the normal (13.75+1.71) and standard drug group (12.50+0.58). CRP value
also show's a non significant increase for the plant extract group (1.
(p<0.05) 02+0.89) when compared to the normal (0.97+0.15) and standard drug
group (0.84+0.14). The results demonstrated that the methanoli leaf extract of
A. boonei has immunosuppresive effect which is shown by the increase in
anti-inflammatory and immunosuppresive cytokine (IL-10) expression.
MACDONALD, C (2023). Immunomodulatory Effect Of Methanol Leaf Extract Of Alstonia Boonei On Albino Wistar Rats:- Okafor, MacDonald .C.. Repository.mouau.edu.ng: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/immunomodulatory-effect-of-methanol-leaf-extract-of-alstonia-boonei-on-albino-wistar-rats-okafor-macdonald-c-7-2
CHIBUEZE, MACDONALD. "Immunomodulatory Effect Of Methanol Leaf Extract Of Alstonia Boonei On Albino Wistar Rats:- Okafor, MacDonald .C." Repository.mouau.edu.ng. Repository.mouau.edu.ng, 19 Oct. 2023, https://repository.mouau.edu.ng/work/view/immunomodulatory-effect-of-methanol-leaf-extract-of-alstonia-boonei-on-albino-wistar-rats-okafor-macdonald-c-7-2. Accessed 24 Nov. 2024.
CHIBUEZE, MACDONALD. "Immunomodulatory Effect Of Methanol Leaf Extract Of Alstonia Boonei On Albino Wistar Rats:- Okafor, MacDonald .C.". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 19 Oct. 2023. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/immunomodulatory-effect-of-methanol-leaf-extract-of-alstonia-boonei-on-albino-wistar-rats-okafor-macdonald-c-7-2 >.
CHIBUEZE, MACDONALD. "Immunomodulatory Effect Of Methanol Leaf Extract Of Alstonia Boonei On Albino Wistar Rats:- Okafor, MacDonald .C." Repository.mouau.edu.ng (2023). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/immunomodulatory-effect-of-methanol-leaf-extract-of-alstonia-boonei-on-albino-wistar-rats-okafor-macdonald-c-7-2