Growth Inhibitory Effect Of Neem (Azadirachta Indicd) Leaf Extract On Klebsiella Species, Salmonella Species, And Staphyloccocus Aureus :- Odoemelam, Harmony
ABSTRACT
The present study was carried out to screen and
evaluate antimicrobial activity of leaf extract of ' Azadirachta indica.
Ethanol and aqueous extracts ofleaves ofA. indica (Neem) were tested against
Klebsiella species, Salmonella species, and Staphyloccocus aureus which are
known to be resistant to various antibiotics. Neem leaves which were used
during this study were collected from Micheal Okpara University of Agriculture,
Umudike. The efficiency of the extracts was studied and determined by applying
different extract concentrations onto the three test organisms using the agar
well dilution method.In this study, the in vitro effect of extracts of Neem
(Azadirachta indica) leaves on Klebsiella species, Salmonella species, and
Staphyloccocus aureus were examined respectively. Ethanol and aqueous extracts
were prepared from both dry and fresh Neem leaves. The susceptibility oftested
bacteria to both extracts was determined by measuring the diameter of
inhibition zones formed around agar wells in different agar plates prepared.
From the analysis, the results showed that fresh Neem leaf extracts were found
to be the most efficient, showing much inhibitory effect on Klebsiella species,
Salmonella species and Staphylococcus aureus compared to dry leaf extracts. The
ethanol extracts were more effective than aqueous extracts in all cases.
S.aureus was- the bacterium most susceptibly affected by these neem leaf
extracts used, while Klebsiella species and Salmonella species showed lower
susceptibility to all the extracts. The results showed that the effectiveness
of the extracts was dependent on the concentration used thus the increase in
extract concentration increased the inhibition zone.
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HARMONY (2025). Growth Inhibitory Effect Of Neem (Azadirachta Indicd) Leaf Extract On Klebsiella Species, Salmonella Species, And Staphyloccocus Aureus :- Odoemelam, Harmony. Repository.mouau.edu.ng: Retrieved Dec 05, 2025, from https://repository.mouau.edu.ng/work/view/growth-inhibitory-effect-of-neem-azadirachta-indicd-leaf-extract-on-klebsiella-species-salmonella-species-and-staphyloccocus-aureus-odoemelam-harmony-7-2
HARMONY. "Growth Inhibitory Effect Of Neem (Azadirachta Indicd) Leaf Extract On Klebsiella Species, Salmonella Species, And Staphyloccocus Aureus :- Odoemelam, Harmony" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 04 Dec. 2025, https://repository.mouau.edu.ng/work/view/growth-inhibitory-effect-of-neem-azadirachta-indicd-leaf-extract-on-klebsiella-species-salmonella-species-and-staphyloccocus-aureus-odoemelam-harmony-7-2. Accessed 05 Dec. 2025.
HARMONY. "Growth Inhibitory Effect Of Neem (Azadirachta Indicd) Leaf Extract On Klebsiella Species, Salmonella Species, And Staphyloccocus Aureus :- Odoemelam, Harmony". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 04 Dec. 2025. Web. 05 Dec. 2025. < https://repository.mouau.edu.ng/work/view/growth-inhibitory-effect-of-neem-azadirachta-indicd-leaf-extract-on-klebsiella-species-salmonella-species-and-staphyloccocus-aureus-odoemelam-harmony-7-2 >.
HARMONY. "Growth Inhibitory Effect Of Neem (Azadirachta Indicd) Leaf Extract On Klebsiella Species, Salmonella Species, And Staphyloccocus Aureus :- Odoemelam, Harmony" Repository.mouau.edu.ng (2025). Accessed 05 Dec. 2025. https://repository.mouau.edu.ng/work/view/growth-inhibitory-effect-of-neem-azadirachta-indicd-leaf-extract-on-klebsiella-species-salmonella-species-and-staphyloccocus-aureus-odoemelam-harmony-7-2