Growth Inhibitory Effect Extract On Klebsiella of Neem (Azadirachta Indica) Leaf A Species, Salmonella Species, And Staphyloccocus Aureus:- Odoemelam, Harmony
ABSTRACT
The present
study was carried out to screen and evaluate antimicrobial activity of leaf
extract of Azadirachta indica. Ethanol and aqueous extracts of leaves of A.
indica (Neem) were tested against Klebsiella species, Salmonella species, and
Staphyloccocus aureus which are known to be resistant to various antibiotics.
Neem leaves which were used during this study were collected from Micheal
Okpara University of Agriculture, Umudike. The efficiency of the extracts was
studied and determined by applying different extract concentrations onto the
three test organisms using the agar well dilution method.In this study, the in
vitro effect of extracts of Neem (Azadirachta indica) leaves on Klebsiella
species, Salmonella species, and Staphyloccocus aureus were examined
respectively. Ethanol and aqueous extracts were prepared from both dry and
fresh Neem leaves. · The susceptibility of tested bacteria to both extracts was
detcnnined by measuring the diameter of inhibition zones formed around agar
wells in different agar plates prepared. From the analysis, the results showed
that fresh Neem leaf extracts were found to be the most efficient, showing much
inhibitory effect on Klebsie/la species, Salmonella species and Staphylococcus
aureus compared to dry leaf extracts. The ethanol extracts were more effective
than aqueous extracts in all cases. S.aureus was the bacterium most susceptibly
affected by these neem leaf extracts used, while Klebsiella species and
Salmonella species showed lower susceptibility to all the extracts. The results
showed that the effectiveness of the extracts was dependent on the
concentration used thus the increase in extract concentration increased the
inhibition zone.
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HARMONY (2025). Growth Inhibitory Effect Extract On Klebsiella of Neem (Azadirachta Indica) Leaf A Species, Salmonella Species, And Staphyloccocus Aureus:- Odoemelam, Harmony. Repository.mouau.edu.ng: Retrieved Oct 30, 2025, from https://repository.mouau.edu.ng/work/view/growth-inhibitory-effect-extract-on-klebsiella-of-neem-azadirachta-indica-leaf-a-species-salmonella-species-and-staphyloccocus-aureus-odoemelam-harmony-7-2
HARMONY. "Growth Inhibitory Effect Extract On Klebsiella of Neem (Azadirachta Indica) Leaf A Species, Salmonella Species, And Staphyloccocus Aureus:- Odoemelam, Harmony" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 29 Oct. 2025, https://repository.mouau.edu.ng/work/view/growth-inhibitory-effect-extract-on-klebsiella-of-neem-azadirachta-indica-leaf-a-species-salmonella-species-and-staphyloccocus-aureus-odoemelam-harmony-7-2. Accessed 30 Oct. 2025.
HARMONY. "Growth Inhibitory Effect Extract On Klebsiella of Neem (Azadirachta Indica) Leaf A Species, Salmonella Species, And Staphyloccocus Aureus:- Odoemelam, Harmony". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 29 Oct. 2025. Web. 30 Oct. 2025. < https://repository.mouau.edu.ng/work/view/growth-inhibitory-effect-extract-on-klebsiella-of-neem-azadirachta-indica-leaf-a-species-salmonella-species-and-staphyloccocus-aureus-odoemelam-harmony-7-2 >.
HARMONY. "Growth Inhibitory Effect Extract On Klebsiella of Neem (Azadirachta Indica) Leaf A Species, Salmonella Species, And Staphyloccocus Aureus:- Odoemelam, Harmony" Repository.mouau.edu.ng (2025). Accessed 30 Oct. 2025. https://repository.mouau.edu.ng/work/view/growth-inhibitory-effect-extract-on-klebsiella-of-neem-azadirachta-indica-leaf-a-species-salmonella-species-and-staphyloccocus-aureus-odoemelam-harmony-7-2