GC-MS Characterization of Bioactive Compound in the Leave of Azadirachta Indica (Neem) and their Anti-Microbial Properties:- Chikwendu, Vincent E.
ABSTRACT
This research
investigated the bioactive compounds present in the leave of Azadirachta indica
(Neem). The experimental work presented in this project is divided into 3 major
parts. The GCMS analysis of the chloroform plant extract shows the presence of
32 bioactive components followed by evaluation of the anti-microbial profile of
the crude extract on selected microorganism such as Salmonella species, Proteus
mirabilis, Staphylococcus aureus and Streptococcus pyogene. 1 hcse bacterial
stains were selected due to its ethno-medicinal ability of the plant. The crude
extract showed a strong antimicrobial effect against all tested bacterial
strains at all concentration. The zone of inhibition was more pronounced in
Salmonella species (8.2 mm) and least pronounced at Proteus mirabilis (2.0 mm)
using different dilution factors: 8.0 mg/ml, 4.0mg/ml and 2.0 mg/ml
respectively. Lastly, vitamins assay which showed Vitamin Bi (38.05±0.45 mg/100
g), Vitamin B2 (34.50±1.10 mg/100 g), Vitamin C (53.40±3.8 mg/100 g) and
Vitamin E (10.85 ± 0.65).
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CHIKWENDU, C (2023). GC-MS Characterization of Bioactive Compound in the Leave of Azadirachta Indica (Neem) and their Anti-Microbial Properties:- Chikwendu, Vincent E.. Repository.mouau.edu.ng: Retrieved Nov 23, 2024, from https://repository.mouau.edu.ng/work/view/gc-ms-characterization-of-bioactive-compound-in-the-leave-of-azadirachta-indica-neem-and-their-anti-microbial-properties-chikwendu-vincent-e-7-2
CHIKWENDU, CHIKWENDU. "GC-MS Characterization of Bioactive Compound in the Leave of Azadirachta Indica (Neem) and their Anti-Microbial Properties:- Chikwendu, Vincent E." Repository.mouau.edu.ng. Repository.mouau.edu.ng, 01 Nov. 2023, https://repository.mouau.edu.ng/work/view/gc-ms-characterization-of-bioactive-compound-in-the-leave-of-azadirachta-indica-neem-and-their-anti-microbial-properties-chikwendu-vincent-e-7-2. Accessed 23 Nov. 2024.
CHIKWENDU, CHIKWENDU. "GC-MS Characterization of Bioactive Compound in the Leave of Azadirachta Indica (Neem) and their Anti-Microbial Properties:- Chikwendu, Vincent E.". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 01 Nov. 2023. Web. 23 Nov. 2024. < https://repository.mouau.edu.ng/work/view/gc-ms-characterization-of-bioactive-compound-in-the-leave-of-azadirachta-indica-neem-and-their-anti-microbial-properties-chikwendu-vincent-e-7-2 >.
CHIKWENDU, CHIKWENDU. "GC-MS Characterization of Bioactive Compound in the Leave of Azadirachta Indica (Neem) and their Anti-Microbial Properties:- Chikwendu, Vincent E." Repository.mouau.edu.ng (2023). Accessed 23 Nov. 2024. https://repository.mouau.edu.ng/work/view/gc-ms-characterization-of-bioactive-compound-in-the-leave-of-azadirachta-indica-neem-and-their-anti-microbial-properties-chikwendu-vincent-e-7-2