ABSTRACT
Vitex doniana
is used in herbal medicine for the treatment of diseases and infection such as
wounds, burns, epilepsy, diarrhea and diabetes. These claims are yet to be
fully documented through scientific evidence, hence the uncovering of the
secondary metabolites present in the leaves, stem and root barks of V. doniana and assessment of their
antioxidant, antimicrobial and antidiabetic potentials. Phytochemicals,
vitamins, minerals and proximate compositions were determined using standard
methods. GC/MS technique was employed in the determination of the volatile
constituents of the chloroform crude extracts of leaves, stem and root barks of
V. doniana. The overall range of
bioactive component range were for alkaloids (1.68-6.39%), flavonoid
(2.18-5.02%), saponin (0.86-4.12%), tannin (0.58-2.50%) and phenol
(0.293-2.20%). The plant vitamins content comprise ascorbic acid
(0.011-0.021%), niacin (0.000128-0.000249%), riboflavin (0.000144-0.000260%),
thiamin (0.000165-0.000497%) and β-caotenoid (0.0566-0.1227%). The plant parts
are good sources of K, Ca, Mg, P, Fe, Cu, Se and Zn. V. doniana leaf crude extract showed a maximum of 0.041trypsin
inhibitory unit (TIU), 25% inhibition against Gram-positive human pathogen, 50%
inhibition with the Gram-negative microorganisms. The antidiabetic experiment
with 25 albino rats showed no significant change in the induced diabetes after
prolonged period treatment, though there was weight gain. By GC/MS analyses the
compounds identified were thirteen from the leaves, nine from the stem bark and
six from the root bark in the class of alkane hydrocarbon, tocopherols,
alkaloids, ether, steroids, ester of unsaturated fatty acids and sulphur
compounds all of which possess physiological uses, for example, [6]
11-[Cyclohexyl ethanamide] Olean 9/11, 13/14-diene, revealed in its
fragmentation pattern the amide function responsible for activity. The
identified compounds revealed bio-protective potentials of V .doniana . In conclusion the leaves, stem bark and root bark of V. doniana have potentials for pharmacological
and physiological functions for good eye health maintenance, treatment of
diabetes, fertility, postpartum bleeding, infection, anemia, and immunity owing
to high value of ascorbic acid in synergy with α-tocopherol and selenium
OKEZIE, O (2024). GC-MS Characterization and Bioprotective Potentials of Secondary Metabolites from the Leaves, Stem Bark and Root Bark of Vitex doniana Sweet (Verbenaceae):- Okwum, Okezie. Repository.mouau.edu.ng: Retrieved Oct 30, 2024, from https://repository.mouau.edu.ng/work/view/gc-ms-characterization-and-bioprotective-potentials-of-secondary-metabolites-from-the-leaves-stem-bark-and-root-bark-of-vitex-doniana-sweet-verbenaceae-okwum-okezie-7-2
OKEZIE, OKEZIE. "GC-MS Characterization and Bioprotective Potentials of Secondary Metabolites from the Leaves, Stem Bark and Root Bark of Vitex doniana Sweet (Verbenaceae):- Okwum, Okezie" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 25 Apr. 2024, https://repository.mouau.edu.ng/work/view/gc-ms-characterization-and-bioprotective-potentials-of-secondary-metabolites-from-the-leaves-stem-bark-and-root-bark-of-vitex-doniana-sweet-verbenaceae-okwum-okezie-7-2. Accessed 30 Oct. 2024.
OKEZIE, OKEZIE. "GC-MS Characterization and Bioprotective Potentials of Secondary Metabolites from the Leaves, Stem Bark and Root Bark of Vitex doniana Sweet (Verbenaceae):- Okwum, Okezie". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 25 Apr. 2024. Web. 30 Oct. 2024. < https://repository.mouau.edu.ng/work/view/gc-ms-characterization-and-bioprotective-potentials-of-secondary-metabolites-from-the-leaves-stem-bark-and-root-bark-of-vitex-doniana-sweet-verbenaceae-okwum-okezie-7-2 >.
OKEZIE, OKEZIE. "GC-MS Characterization and Bioprotective Potentials of Secondary Metabolites from the Leaves, Stem Bark and Root Bark of Vitex doniana Sweet (Verbenaceae):- Okwum, Okezie" Repository.mouau.edu.ng (2024). Accessed 30 Oct. 2024. https://repository.mouau.edu.ng/work/view/gc-ms-characterization-and-bioprotective-potentials-of-secondary-metabolites-from-the-leaves-stem-bark-and-root-bark-of-vitex-doniana-sweet-verbenaceae-okwum-okezie-7-2