ABSTRACT
This
study was aimed to evaluate the intestinal and serum amylase activity of female
Wistar rats administered with butanol extract of Vernonia amygdalina and high
fat diet. Twenty (20)Female Wistar rats weighing between 95-112g were used for
this study. The animals were kept in metal cages and left under laboratory
conditions for two weeks for acclimatization, with free access to growers mash
and water. The rats were randomly assigned into five[5] groups offour[4] rats
each, Group 1 was the control group fed with basal diet and water While the
Group 2 were fed with high fat diet only, Group 3 were treated high fat diet
and low dose of lOOmg/kg Vernonia amygdalina while Group 4 was treated with
high fat diet and high dose of 200mg/kg Vernonia amygdalina,the Group 5 was the
standard group treated with high fat diet and orlistat, they were treated for 5
weeks and were sacrificed, collecting the blood sample by cardiac puncture for
laboratory analysis. The result showed a significant increase (p<0.05) in
the intestinal lipase and serum amylase in the groups treated with the High fat
Diet alone, HFD + V. amygdalina (lOOmg/kg), HFD+ V. amygdalina (200mg/kg) and
HFD + the standard drug (Orlistat). Similarly, the serum amylase (which is an
enzyme or special protein that helps in the digestion of carbohydrate) showed
similar trend with the intestinal lipase, with significant rise in the wistar
rats fed HFD alone compared with the rats that received the treatment, and the
normal control. The rats fed HFD alone had the highest (p<0.05) mean serum
amylase value (205.25±0.45 U/L), followed by those that received the HFD +
Orlistat (175.96±0.19 U/L) and the extract at high dose (175.24±0.55
U/L).Therefore, to achieve an appreciable anti-hyperlipidemia activity using
butanol extract of V. amygdalina extract, the low dose (lOOmg/kg) is highly
recommended
-- (2024). Evaluation Of The Intestinal And Serum Amylase Activity Of Female Wistar Rats Administered With Butanol Extract Of Vernonia amygdalina And High. Fat Diet:- Agu, Chioma A . Repository.mouau.edu.ng: Retrieved Dec 09, 2024, from https://repository.mouau.edu.ng/work/view/evaluation-of-the-intestinal-and-serum-amylase-activity-of-female-wistar-rats-administered-with-butanol-extract-of-vernonia-amygdalina-and-high-fat-diet-agu-chioma-a-7-2
--. "Evaluation Of The Intestinal And Serum Amylase Activity Of Female Wistar Rats Administered With Butanol Extract Of Vernonia amygdalina And High. Fat Diet:- Agu, Chioma A " Repository.mouau.edu.ng. Repository.mouau.edu.ng, 14 Oct. 2024, https://repository.mouau.edu.ng/work/view/evaluation-of-the-intestinal-and-serum-amylase-activity-of-female-wistar-rats-administered-with-butanol-extract-of-vernonia-amygdalina-and-high-fat-diet-agu-chioma-a-7-2. Accessed 09 Dec. 2024.
--. "Evaluation Of The Intestinal And Serum Amylase Activity Of Female Wistar Rats Administered With Butanol Extract Of Vernonia amygdalina And High. Fat Diet:- Agu, Chioma A ". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 14 Oct. 2024. Web. 09 Dec. 2024. < https://repository.mouau.edu.ng/work/view/evaluation-of-the-intestinal-and-serum-amylase-activity-of-female-wistar-rats-administered-with-butanol-extract-of-vernonia-amygdalina-and-high-fat-diet-agu-chioma-a-7-2 >.
--. "Evaluation Of The Intestinal And Serum Amylase Activity Of Female Wistar Rats Administered With Butanol Extract Of Vernonia amygdalina And High. Fat Diet:- Agu, Chioma A " Repository.mouau.edu.ng (2024). Accessed 09 Dec. 2024. https://repository.mouau.edu.ng/work/view/evaluation-of-the-intestinal-and-serum-amylase-activity-of-female-wistar-rats-administered-with-butanol-extract-of-vernonia-amygdalina-and-high-fat-diet-agu-chioma-a-7-2