ABSTRACT
Vegetables
are widely used as herbal remedies for combating liver diseases. This study
evaluated the phyto contents and in vitro
antioxidant capacity of various solvent fraction of the crude extract as
well as some biochemical changes following exposure to the hydro-alcohol
extract of Amaranthus hybridus in
thioacetamide- induced hepatic damage in rats. The plant sample was extracted
with 70% ethanol. Preliminary photochemistry and in vitro antioxidant potential of the plant extract was performed
using standard methods. The
hepatoprotective effect of HAAH extract was measured in rat model of
thioacetamide- induced liver damage for 70 days. Animals were divided into six
(6) groups, and were exposed to thioacetamide (300mg/l) in drinking water
throughout experimental period, except group that served as control. Liver
function status test performed to access the possible effect of HAAH on liver
damage. Oxidative stress conditions were measured using malondialdehyde, nitric
oxide and glutathione levels. The effects protective activity of HAAH on free
radical scavenging were assayed using enzymatic antioxidants. Histology of the liver
tissue was performed to access if the extract protected liver architecture from
damage. Fractionation of crude plant extract was achieved using solvent
partitioning system, and various fractions assayed for antioxidant potential to
confirm most potent fraction. The most active fraction was characterised using
GC-MS spectrometry. Result showed that the plant extract was rich in phenolic
compounds (140.12+0.01 GAEequ.mg/g), flavonoids (3.39+0.02 mgQE/g)
and Vitamin C (2.86+0.01mg/100g). Amino acid profiling of the plant
extract showed that the extract contained 16 amino acids, which include aromatic
amino acids phenylalanine (3.34%), tyrosine (6.08%) and many essential amino
acids. In vitro antioxidant studies showed a dose dependent activity for the plant
extract that was comparable to standard vitamin c. Results for liver function status
assay showed significant (p<0.05) reduction in the liver enzymes, increase
in total proteins in the groups administered HAAH compared to negative control.
There was significant (p<0.05) increase in enzymatic antioxidant activities in
liver homogenates in group administered the plant extract compared to negative
control. MDA was significantly (p<0.05) lower in the group treated with
plant extract when compared to negative control, while nitric oxide inhibition and
GSH concentration were higher in group treated with the plant extract compared
to negative control. Histology findings corroborated result obtained from the
liver function status assays. Methanol fraction of crude extract showed highest
antioxidant potential, and GC-MS profiling of the methanol fraction showed 6
compounds having benzene ring backbone, like those found in most phenolic
acids. From the result of this study, it can be concluded that methanol
fraction of HAAH contains compounds that may be responsible for the observed hepatoprotective
and antioxidant properties.
EJIOFOR, E (2022). Effects Of Hydro-Alcohol Extract Of Green Amaranth (Amaranthus Hybridus Linn.) Leaves On Biochemical Indices Of Thioacetamide-Induced Hepatic Damage In Rats. Repository.mouau.edu.ng: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/effects-of-hydro-alcohol-extract-of-green-amaranth-amaranthus-hybridus-linn-leaves-on-biochemical-indices-of-thioacetamide-induced-hepatic-damage-in-rats-7-2
EJIOFOR, EJIOFOR. "Effects Of Hydro-Alcohol Extract Of Green Amaranth (Amaranthus Hybridus Linn.) Leaves On Biochemical Indices Of Thioacetamide-Induced Hepatic Damage In Rats" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 20 Oct. 2022, https://repository.mouau.edu.ng/work/view/effects-of-hydro-alcohol-extract-of-green-amaranth-amaranthus-hybridus-linn-leaves-on-biochemical-indices-of-thioacetamide-induced-hepatic-damage-in-rats-7-2. Accessed 24 Nov. 2024.
EJIOFOR, EJIOFOR. "Effects Of Hydro-Alcohol Extract Of Green Amaranth (Amaranthus Hybridus Linn.) Leaves On Biochemical Indices Of Thioacetamide-Induced Hepatic Damage In Rats". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 20 Oct. 2022. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/effects-of-hydro-alcohol-extract-of-green-amaranth-amaranthus-hybridus-linn-leaves-on-biochemical-indices-of-thioacetamide-induced-hepatic-damage-in-rats-7-2 >.
EJIOFOR, EJIOFOR. "Effects Of Hydro-Alcohol Extract Of Green Amaranth (Amaranthus Hybridus Linn.) Leaves On Biochemical Indices Of Thioacetamide-Induced Hepatic Damage In Rats" Repository.mouau.edu.ng (2022). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/effects-of-hydro-alcohol-extract-of-green-amaranth-amaranthus-hybridus-linn-leaves-on-biochemical-indices-of-thioacetamide-induced-hepatic-damage-in-rats-7-2