ABSTRACT

This study was carried out to check the effect of soybean {Glycine max) and black Velvet (Dialium gnineense) on some antioxidant enzymes and vitamins in male wistar rats. Twenty (20) male wistar rats were divided randomly into four groups. Group 1 served as the normal (control) and they received only commercial rat feed and drinking water. Group 2 received soybean {Glycine max) and they also received the commercial rat feed and drinking water. Group 3 received black velvet and they also received the commercial rat feed and drinking water. Group 4 received soybean {Glycine max) and black velvet with little commercial rat feed and drinking water. Changes in their body weights were recorded once in a week throughout the duration of the experiment. Antioxidant assays and level of lipid peroxidation were investigated. The result of the study indicated that D.guineense showed non-significant (p>0.05) increase in catalase, malondialdehyde (MDA) and vitamin C, but when compared to the normal group a significant (p <0.05) increase in malondialdehyde (MDA), vitamin E and catalase activity was observed when mixed with soybeans. D.guineense with feed and when mixed with soybean showed a significant (p <0.05) increase in vitamin C activity when compared with soybean with feed. D.guineense with feed showed a high significant (p <0.05) increase in vitamin E activity when compared to soybean with feed but showed a significant (p <0.05) decrease when compared with D.guineese with soybean. Soybeans showed significant (p <0.05) increase effect on the activity of malondialdehyde (MDA) and catalase in group 2 and grroup 4 when compared to the normal group. The mixture ofsoybean and D.guineense showed a significant (p <0.05) increase in catalase and vitamin E activities when compared to D.guineense feed and the normal group. Soybean showed a high significant (p <0.05) increase in malondialdehyde (MDA) activity when compared with D.guineese with soybean and D.guineense with feed but when mixed with D. guineense it showed a significant (p <0.05) increase when compared to D.guineense with feed and in catalase activity soybean showed a significant (p <0.05) increase when compared to D.guineense with feed and a significant (p <0.05) decrease when compared to D. guineense with soybean. In SOD activity soybean, D. guineense and D.guineese with soybean showed non-significant (p <0.05) increase when compared to the normal control.