ABSTRACT

The study was aimed at investigating the effects of methanol extract of ass stasia gangetica leaves on selected biochemical parameters of the kidney of a monosodium glutamate challenged wistar albino rats. A total of 36 male wistar albino rats and 18 mice were used for this study. The eighteen male Wistar albino mice were divided into two major groups of nine (9) mice each. Each of the two groups were further divided into three having three mice each. The experimental rats were divided into nine groups with four rats in each group. The study was conducted following standard analytical protocols. The parameters reviewed were serum electrolyte, creatinine, urea, urid acid, serum bicarbonate ions, serum potassium ions, serum chloride ions, and sodium ions in normal, control, positive control and experimental rats respectively. The normal control rats received 2ml/kg body weight of distilled water for 14 days, while group 2 rats received a suspension of monosodium glutamate, 8g/kg body weight on the seventh and fourteenth day. Group 3 received 8g/kg body weight of monosodium glutamate and were treated with 200mg/kg bodyweight of Silymarine for 14 days. Group 4 received 200mg/kg bodyweight of methanol extract ofAsyslasia gengalicu leaves for 14 days, received 500mg/kg bodyweight of methanol extract of Asystasiu yenf'cilicu for 14 days. Group 6 received 200mg/kg bodyweight of methanol extract ofAsyslasici gengaHea for 7 days. After 30 minutes on 7lh and 14,h day respectively monosodium glutamate was administered. They continued to receive the extracts for the next 7 days. Group 7 received 500mg/kg bodyweight of the extract for 7 days. After 30 minutes on day 7lh and 14th respectively monosodium glutamate suspension was administered. They continued receiving the extract for the next 7 days. Group 8 received a suspension ofmonosodium glutamate on day one and day two and 14 respectively. Treatment with 200mg/kg bodyweight of the extract commenced after 24 hours for 14 days. In the group 2 rats, a significant (1’0.05) increased in the examined kidney functional indices was observed. This means that the extract poses no danger to the kidney. Group 3 showed a significant (1’0.05) decreased compared to group 2. In group 6 and 7, there was a significant (1’0.05) compared to group 2. From the value, the extract that was first received posed no danger to the kidney. After the monosodium glutamate was administered, the increased noticed was however not as bad as that of group 2. The extract ameliorate the later adverse effect of the monosodium glutamate that was given. In group 8 and 9. there was a significant (1’0.05) decrease, the extract seemed to restore the kidnej to the normal especially when administered in doses. From the above, it is imperative to establish that monosodium may cause an adverse effect on the renal function which might be due to oxidation stress. The effect ofthe extract restoring in renal function could be attributed to the extract possessing sufficient antioxdant component. Thus, it is concluded that methanol extract ofAsystasia gcingelica induced challenged wistar albino rat. The findings from this study showed that administration of Monosodium glutamate (MSG) caused an increased in the levels of serum electrolyte ions concentration (Chloride, potassium, sodium, calcium and bicarbonate ions).