Cyanogenic Potential And Activity Of Partially Purified B-Glucosidase Of Cassava Cultivar TMS 1089A
ABSTRACT
This work aimed to determine the cyanogenic potential of cassava
cultivar TMS 1089A, using the protocol- B method and also the activity
of the partially purified enzyme which was extracted from the cassava
peel using ammonium sulphate precipitation method. The result of the
analysis showed that a higher total cyanide distribution was found in the
dried leaves of the cassava cultivar with 268.092ppm, followed by the
fresh peel (138.204ppm), fresh leaves (79.990ppm), fresh stem
(43.560ppm), fresh pulp(29.700ppm), dried pulp (17.820ppm) and then
the dried peel (5.148ppm) which has the lowest cyanide content. The
partially purified enzyme has an activity of 4.Ounits/ml and specific
activity of O.63units/mg protein. The analysis for Carotenoid gave a
value of 6.514mgJg and protein content was 6.36mg/ml. The partially
purified enzyme showed high degree of hydrolytic activity towards the
cyanogenic glycosides of cassava and products of some other root tubers
than when compared to 3-glucosidase of activity 3.Ounit/ml.
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JOSEPH, C (2021). Cyanogenic Potential And Activity Of Partially Purified B-Glucosidase Of Cassava Cultivar TMS 1089A . Repository.mouau.edu.ng: Retrieved Nov 23, 2024, from https://repository.mouau.edu.ng/work/view/cyanogenic-potential-and-activity-of-partially-purified-b-glucosidase-of-cassava-cultivar-tms-1089a-7-2
CHIDINMA, JOSEPH. "Cyanogenic Potential And Activity Of Partially Purified B-Glucosidase Of Cassava Cultivar TMS 1089A " Repository.mouau.edu.ng. Repository.mouau.edu.ng, 04 Jun. 2021, https://repository.mouau.edu.ng/work/view/cyanogenic-potential-and-activity-of-partially-purified-b-glucosidase-of-cassava-cultivar-tms-1089a-7-2. Accessed 23 Nov. 2024.
CHIDINMA, JOSEPH. "Cyanogenic Potential And Activity Of Partially Purified B-Glucosidase Of Cassava Cultivar TMS 1089A ". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 04 Jun. 2021. Web. 23 Nov. 2024. < https://repository.mouau.edu.ng/work/view/cyanogenic-potential-and-activity-of-partially-purified-b-glucosidase-of-cassava-cultivar-tms-1089a-7-2 >.
CHIDINMA, JOSEPH. "Cyanogenic Potential And Activity Of Partially Purified B-Glucosidase Of Cassava Cultivar TMS 1089A " Repository.mouau.edu.ng (2021). Accessed 23 Nov. 2024. https://repository.mouau.edu.ng/work/view/cyanogenic-potential-and-activity-of-partially-purified-b-glucosidase-of-cassava-cultivar-tms-1089a-7-2