ABSTRACT

Mushroom is one of the most suitable fungal organisms for producing protein rich food from various agro-wastes without composting. The present study was conducted to evaluate and compare the effect of different Agricultural waste on growth, yield, proximate, vitamins, Amino acid profile, micro and macro-nutrients of Pleurotus ostreatus var jlorida and P.pulmonarius were cultivated on different agro-waste viz Treculia african(TA), Pakia biglobosa (PB)and Vigna substerraneae (VS) pod alone and in combination of I: I ratio. Factorial in complete randomized design(CRD) was used to evaluate the results on different substrates. Analysis of variance (ANOVA) was employed to text for the overall significance of data while the least significance difference (LSD) test was used to compare the difference among nthe two varieties. The results showed maximum yield of P. ostreatus var florida was recorded on Vigna mixed with pakia pod 77.30g/kg (7.73BE) whereas minimum value was recorded on Treculia alone (21.80g/kg 2.18%BE). Maximum yield of P. pulmonarius was recorded Treculia pod alone 83.67g/kg (8.37%BE) followed by combination of Vigna and Pakia pod 82.97g/kg (8.30%BE) which varied significantly from other substrates (P < 0.05).Interaction studies show that P. pulmonarius recorded better economic yield and biological efficiency over P, ostreatus var florida, substrate Vs+Pb gave better yield of both mushroom species. Pileus diameter and stripe length did not vary on substrates (P > 0.05% )but varied on the two species at S% level of difference. Proximate analysis indicated the presence of protein, dietary fiber, ash fat and carbohydrate. Maximum crude protein (36.07), dietary fibre (6.42) and ash (7.41) were observed when P. pulmonarius was cultivated on Vigna pod alone and minimum fibre, ash carbohydrates was observed on Treculia combined with Vigna pod. Fat and oil content on Treculia pod alone xii was higher but not significantly different from those produced on other substrates. Vigna: Pakia pod showed maximum ash and protein content of P, ostreatus var florida. Interaction study shows that P. ostreatus var jlorida showed better protein, ash, fibre, carbohydrate and moisture than P.pulmonarius. Mushroom sample was found to contain variable minerals. Result revealed that phosphorus had the highest value in all the minerals sampled and it values ranges between 42.7lg/100gto 60.83mg/100g while calcium recorded the least value of 0.267mg/100g to 2.l 93mg/l 00g followed by magnesium, it value ranged between 2.33mg/I 00g to 4.45mg/I 00g. Interaction studies showed that P. pulmonarius was recorded best in mineral content across the substrates. Mineral content of P. ostreatus and P. pulmonarius is not significantly different from each other at 5% level of significance ( P > 0.05) but very highly significant at 0.00 I level of difference( P < 0.001) combination of Treculia and Vigna pod yielded the highest vitamin value on P. pulmonarius (P < 0.001 ). The importance of a foodstuff as source of protein depend on the kind of amino acid that form the protein .Amino acid profile showed that P. ostreatus var florida and P. pulmonarius are rich in essential and non-essential amino acid, glutamic acid, aspartie acid, throenine and valine. The result shows that the substrates did not have significant effect on the amino acid profile (P > 0.05).