ABSTRACT
Pigeon pea (Cajanus cajan (L.) Millspaugh) is an
important legume known for its nutritional value. In the present study, seeds
of pigeon pea accessions (Brown and Cream colours) were treated with different
concentrations of ethyl methane sulphonate. Seven different concentrations were
prepared (0.00 %, 0.01 %, 0.02 %, 0.03 %, 0.04 %. 0.05 %. 0.06 % and 0.07 %
w/v). Field experiment was conducted using the same EMS concentrations for M,
and M2 generations. The screen house experiment was set up in a complete
randomized design and field experiment was set up in a randomized complete
block design respectively. Growth data were collected at 3, 6, 9 and 12 weeks
after planting in M| and M2 generations whereas yield data were collected at
maturity. The results obtained showed that the effect of the mutagen treatments
on germination and survival percentages were highly significant (p<0.001).
The results showed that 50 % germination was recorded under treatment 0.05 %
EMS in the Brown colour accession and 0.04 % EMS in the Cream colour. These
concentrations are therefore considered as the LD50 values. The result obtained
showed that the treatments significantly improved the vegetative traits of the
pigeon accessions in both generations. There were significant differences
(P<0.05) between the means of the treated plants when compared to the
control plants. Data obtained from the study showed that treatment 0.04 % EMS
enhanced vegetative performances in the Brown colour accession whereas, in the
Cream accession, 0.03 % EMS induced better performance. The yield performances
of the pigeon pea accessions were significantly improved by the mutagen
treatments. The result of the analysis of variance showed that plants treated
with 0.03 % and 0.04 % EMS produced better yields when compared with the
control plants. The results of the proximate, anti-nutrient and mineral
compositions ofthe plants in Mi and M2 generations showed that the effect of
the mutagen was highly significant (P<0.001) when compared with the control
plants. The anatomical studies revealed an increase in the stomatai index in
plants treated with 0.01 % EMS on the abaxial surface. The root xylem vessels,
thickened more on the higher concentrations (0.03 % - 0.04 % EMS). The cell
walls of plants treated with 0.03 % - 0.04 % EMS were thicker with reduced
intercellular air spaces. The cortical cells of plants treated with 0.01 % -
0.02 % and the control had thinwalled cells with large intercellular air
spaces. Molecular characterization was carried out using a simple sequence
repeat marker for diversity and sodium dodecyl sulphatepolyacrylamide gel
electrophoresis (SDS-PAGE). Ribulose-1,5-biphosphate marker was used in gene
sequencing. Higher polymorphic information value was observed in primer
CZ681993 (0.7122) with several alleles. The results of the present studies have
shown that EMS at 0.03 % and 0.04 % could be used by plant breeders to improve
the agronomic characters of pigeon pea. It is therefore recommended that plant
breeders employ this mutagen in the breeding program of pigeon pea to create
beneficial mutation amidst the ever-increasing human population and food
insecurity
-- (2025). Studies On The Effects Of Ethyl Mjethane Sulphonate (Ems) On Two Pigeon Pea (Cajanus Cajan (L.) Millspaugh) Accessions In Mi And M2 Generations:- Akanwa, Franklin E. Repository.mouau.edu.ng: Retrieved Jul 01, 2025, from https://repository.mouau.edu.ng/work/view/studies-on-the-effects-of-ethyl-mjethane-sulphonate-ems-on-two-pigeon-pea-cajanus-cajan-l-millspaugh-accessions-in-mi-and-m2-generations-akanwa-franklin-e-7-2
--. "Studies On The Effects Of Ethyl Mjethane Sulphonate (Ems) On Two Pigeon Pea (Cajanus Cajan (L.) Millspaugh) Accessions In Mi And M2 Generations:- Akanwa, Franklin E" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 30 Jun. 2025, https://repository.mouau.edu.ng/work/view/studies-on-the-effects-of-ethyl-mjethane-sulphonate-ems-on-two-pigeon-pea-cajanus-cajan-l-millspaugh-accessions-in-mi-and-m2-generations-akanwa-franklin-e-7-2. Accessed 01 Jul. 2025.
--. "Studies On The Effects Of Ethyl Mjethane Sulphonate (Ems) On Two Pigeon Pea (Cajanus Cajan (L.) Millspaugh) Accessions In Mi And M2 Generations:- Akanwa, Franklin E". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 30 Jun. 2025. Web. 01 Jul. 2025. < https://repository.mouau.edu.ng/work/view/studies-on-the-effects-of-ethyl-mjethane-sulphonate-ems-on-two-pigeon-pea-cajanus-cajan-l-millspaugh-accessions-in-mi-and-m2-generations-akanwa-franklin-e-7-2 >.
--. "Studies On The Effects Of Ethyl Mjethane Sulphonate (Ems) On Two Pigeon Pea (Cajanus Cajan (L.) Millspaugh) Accessions In Mi And M2 Generations:- Akanwa, Franklin E" Repository.mouau.edu.ng (2025). Accessed 01 Jul. 2025. https://repository.mouau.edu.ng/work/view/studies-on-the-effects-of-ethyl-mjethane-sulphonate-ems-on-two-pigeon-pea-cajanus-cajan-l-millspaugh-accessions-in-mi-and-m2-generations-akanwa-franklin-e-7-2