Comparative Evaluation On Different Extraction Solvent And Techniques For The Assay Of Antioxidant Activity Of Vernonia Colorata.:- Udoidung, Ubongabasi E
ABSTRACT
This study was designed to evaluate the
different extraction yield and concentration of antioxidants in vemonia
colorata using different extraction technique. Dry leafs of vemonia colorata
were extracted using 50:50 ethanol and methanol mixture using different
extraction technique via Cold water, digital water-bath and shaker, soxhlet and
microwave assisted extraction. The antioxidants such as the total phenolic
content(TPC),total flavonoid content and l,l-diphenyl-2-picryl-hydrazil (DPPH)
radical scavenging were determine spectrophotometrically in all the extract.
Percentage yield using different extraction technique was as follow cold water
(ethanol 16.8%, methanol 20.8%), Digital water-bath and shaker gave(ethanol
19.6%, methanol 17.6%), soxhlet (ethanol 9.2%, methanol 32%) and microwave
assisted (ethanol 24%, methanol 21%). The highest TPC was obtain from the
digital shaker (46.17±2.74) using ethanol solvent. The highest concentration in
total flavonoid content was obtain from soxhlet method and ethanol as solvent
(1062.04±313.80) while the DPPH radical scavenging activity was highest in
ethanol extract obtained in soxhlet method (258.82±26.92). These finding shows
that for antioxidants assays, the use ofsoxhlet method and ethanol solvent
gives high DPPH scavenging activity and Total flavonoid content while digital
shaker and ethanol solvent gives highest ofTPC.
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UBONGABASI, E (2024). Comparative Evaluation On Different Extraction Solvent And Techniques For The Assay Of Antioxidant Activity Of Vernonia Colorata.:- Udoidung, Ubongabasi E. Repository.mouau.edu.ng: Retrieved Dec 22, 2024, from https://repository.mouau.edu.ng/work/view/comparative-evaluation-on-different-extraction-solvent-and-techniques-for-the-assay-of-antioxidant-activity-of-vernonia-colorata-udoidung-ubongabasi-e-7-2
EDWARD, UBONGABASI. "Comparative Evaluation On Different Extraction Solvent And Techniques For The Assay Of Antioxidant Activity Of Vernonia Colorata.:- Udoidung, Ubongabasi E" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 10 Oct. 2024, https://repository.mouau.edu.ng/work/view/comparative-evaluation-on-different-extraction-solvent-and-techniques-for-the-assay-of-antioxidant-activity-of-vernonia-colorata-udoidung-ubongabasi-e-7-2. Accessed 22 Dec. 2024.
EDWARD, UBONGABASI. "Comparative Evaluation On Different Extraction Solvent And Techniques For The Assay Of Antioxidant Activity Of Vernonia Colorata.:- Udoidung, Ubongabasi E". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 10 Oct. 2024. Web. 22 Dec. 2024. < https://repository.mouau.edu.ng/work/view/comparative-evaluation-on-different-extraction-solvent-and-techniques-for-the-assay-of-antioxidant-activity-of-vernonia-colorata-udoidung-ubongabasi-e-7-2 >.
EDWARD, UBONGABASI. "Comparative Evaluation On Different Extraction Solvent And Techniques For The Assay Of Antioxidant Activity Of Vernonia Colorata.:- Udoidung, Ubongabasi E" Repository.mouau.edu.ng (2024). Accessed 22 Dec. 2024. https://repository.mouau.edu.ng/work/view/comparative-evaluation-on-different-extraction-solvent-and-techniques-for-the-assay-of-antioxidant-activity-of-vernonia-colorata-udoidung-ubongabasi-e-7-2