ABSTRACT
Baphia nitida is a medicinal plant of African origin that
belongs to the family of Leguminosae. This plant has diverse and potential
medicinal uses in traditional medicine for treating gastrointestinal problems,
veneral diseases, diabetes, constipation, skin diseases, swollen joints,
cardiac pain, diarrhoeal, painful menstruation and female sterility. This study
was undertaken to investigate the bioactive constituents, vitamin content,
mineral composition and antibacterial properties of chloroform extract of
Baphia nitida leaf. The phytochemical screening of the extract revealed the
presence of alkaloids, polyphenols, flavonoids, saponins, tannins and
glycosides, with polyphenols registering the highest presence. 1-Eicosene was
isolated from the leaves of B. nitida and was characterized using 'Fl, 13 C,
COSY, DEPT, HMBC and FISQC NMR Spectroscopic techniques. Vitamins detected
included thiamin (0.23 8±0.013 mg/bOg), niacin (1.070±0.035 mg/bOg), riboflavin
(0.064±0.001 mg/bOg), ascorbic acid (5.600±0.000 mg/bOg) and f3-carotene
(4.930±0.025 mg/bOg), with ascorbic acid and riboflavin having the highest and
lowest concentrations respectively. Minerals detected included calcium
(3164.5±0.245 mg/bOg), magnesium (1370.3±0.030 mg/i 00g), potassium
(589.7±0.008 mg/bOg), sodium (390.2±0.008 mg/bOg), phosphorus (538.7±0.013
mg/bOg), nitrogen (3045.3±0.035 mg/bOg), iron (1.767±0.165 mg/bOg), zinc
(0.423±0.025 mg/bOg) and copper (0.003±0.000 mg/bOg). Of the minerals detected,
calcium had the highest concentration, while copper had the lowest. The
antibacterial activity at varying concentrations; 100 mg/mi, 50 mg/mi, 40
mg/mi, 20 mg/ml and 10 mg/mi of the extract was investigated against
Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa using agar
well diffusion method. The results of the antimicrobial assay showed that the
extract inhibited the growth of the test organisms in a concentration dependent
manner. The highest growth inhibition was seen against Staphylococcus aureus
and the lowest growth inhibition was against Pseudomonas aeruginosa with MIC of
20.00±0.00 mg/mi and 49.67±4.71 mg/mi respectiveiy. The results of this
research work revealed that Baphia nitida has a lot of phytochemicals which
could be used as raw material by pharmaceutical industries for drug
formulation. The ability of the extract from this plant to exhibit potent
inhibition against these species of bacteria lent a contributing scientific
evidence for the use of this plant in traditional medicine.
IHENETU, U (2021). Chemical Studies And Nmr Characterization Of A Secondary Metabolite Isolated From The Leaves Of Baphia Nitida Lodd. Repository.mouau.edu.ng: Retrieved Nov 23, 2024, from https://repository.mouau.edu.ng/work/view/chemical-studies-and-nmr-characterization-of-a-secondary-metabolite-isolated-from-the-leaves-of-baphia-nitida-lodd-7-2
UZOMA, IHENETU. "Chemical Studies And Nmr Characterization Of A Secondary Metabolite Isolated From The Leaves Of Baphia Nitida Lodd" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 05 Jul. 2021, https://repository.mouau.edu.ng/work/view/chemical-studies-and-nmr-characterization-of-a-secondary-metabolite-isolated-from-the-leaves-of-baphia-nitida-lodd-7-2. Accessed 23 Nov. 2024.
UZOMA, IHENETU. "Chemical Studies And Nmr Characterization Of A Secondary Metabolite Isolated From The Leaves Of Baphia Nitida Lodd". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 05 Jul. 2021. Web. 23 Nov. 2024. < https://repository.mouau.edu.ng/work/view/chemical-studies-and-nmr-characterization-of-a-secondary-metabolite-isolated-from-the-leaves-of-baphia-nitida-lodd-7-2 >.
UZOMA, IHENETU. "Chemical Studies And Nmr Characterization Of A Secondary Metabolite Isolated From The Leaves Of Baphia Nitida Lodd" Repository.mouau.edu.ng (2021). Accessed 23 Nov. 2024. https://repository.mouau.edu.ng/work/view/chemical-studies-and-nmr-characterization-of-a-secondary-metabolite-isolated-from-the-leaves-of-baphia-nitida-lodd-7-2