ABSTRACT

Fifty (50) High Vaginal Swab (HVS) were carefully and aseptically carried from pregnant women with complaint of vaginal discharge. The samples were analyzed using standard microbiological methods. Wet preparation of the samples were examined microscopically and the Swabs were cultured on Sabouraud Dextrose Agar (SDA) plates and incubated at 37oC for 3-4 days. The overall prevalence of Candida albican was Eighten (36%). This investigation recommends good personal hygiene and regular check up for women domiciled in this area since Candida albicans poses a major health challenge to avoid complicated health problems. 

TABLE OF CONTENTS

Title page i

Declaration ii

Certification iii

Dedication iv

Acknowledgements v

Table of contents vi

List of tables viii

Abstract ix

CHAPTER ONE

1.0 Introduction 1

1.2 Aims and objectives 6

CHAPTER TWO

2.0 Literature review    7

2.1 Candidiasis 7

CHAPTER THREE

3.0 Materials and Methods    18

3.1 Collection of Samples 18

3.2 Wet Preparation 18

3.3 Microbiological Analysis 18

3.4 Sterilization 19

3.5 Identification of Bacteria 19

3.6 Gram Staining 19

3.7       Catalase Test 20

3.7.1 Coagulase Test 20

3.7.2 Citrate Test 20

3.7.3 Motility, Indole, Urease Test (MIU) 21

3.7.4 Triple Sugar Iron Test 21

3.7.5 Oxidase Test 22

CHAPTER FOUR

4.0 Results    23

CHAPTER FIVE

5.0 Discussion, Recommendation and Conclusion 27

5.1 Recommendation 28

5.2 Conclusion 28

References