ABSTRACT

Five different drinking water sources were analysed bacteriological. The sources are packaged water (paani), Male hostel tank, Female Hostel tank, stream and spring water. Multiple tubes technique was used for the test and biochemical test was also used to identify the bacteria present. The suspected organism was Enterobacter spp. Following the WHO guideline for potable water, all the samples are not acceptable for drinking.

TABLE OF CONTENT

TITLE PAGE i

CERTIFICATION ii

DEDICATION iii

ACKNOWLEDGEMENT iv

TABLE OF CONTENTS v

LIST OF TABLES vi

ABSTRACT vu

CHAPTER ONE

1.0 INTRODUCTION

CHAPTER TWO

2.0 LITERATURE REVIEW 4

2.1 CAUSE OF WATERPOLLUTION 5

2.2 EFFECTS OF WATER POLLUTiON 5

2.3 EXAMINAT'10N OF DRINKING WATER 6

2.4 CRITERIA FOR THE CHOICE OF INDICATOR

ORGANISM. . 7

2.4.1 COLIFORM BACTERIA 8

2.4.2 FAECAL STREPTOCOCCI 9

2.4.3 CLOSTRIDIUM PERFRINGENS 9

2.4.4 PSEUDOMNAS AERUGINOSA 10

2.4.5 BIFIDOBACTERIA . .• 10

2.5 FAECAL CONCENTRATIONS, DETECTiON AND ENUMERATiON

OF BACTERICAL INDICATORS. Ii

2.6.1.ISTANDARDS FOR THE BACTE:. R1OLOGCALL QUALITY OF

DRINK[NG WATER 12

2.7 WHO GUIDELINES FOR DRINKiNG WATER: 13

CHAPTER THREE

3.0 MATERIALS AND METHODS 14

3.1 ENVIRONMENT OF STUDY 14

3.2 METHOD OF SAMPLING 14

3.3 ANALYTICAL METHODS 15

3.3.1 PRESUMPTIVE TEST 16

3.3.2 CONFIRMATORY TEST 16

3.3.3 COMPLETED TEST 16

3.3.4 BiOCHEMICAL TEST 16

3.3.5 EXAMINATION FOR STRETOCOCCUS FAECALIS

3.3.6 EXAMINATION FOR CLOSTRIDU1UM PERFR1NGRS

3.3.7 PLATE COUNT

CHAPTER FOUR

4.0 RESULTS AND DISCUSSION

4.1 RESULT OF PRESUMPTIVE TEST.

4.2 RESULT OF COMFIRMATORY TEST

4.3 RESULT OF COMPLETED TEST

4.4 RESULT OF BIOCHEMICAL TEST

4.5 RESULT OF STREPTOCOCCUS FAECALIS TEST

4.6 RESULT OF CLOSTRIDIUM PERFRINGENS TEST

4.7 PLATE COUNT RESULT

CONCLUSION

REFERENCE

APPENDIX