Antidiabetic potential of methanol bulb extracts of bulbinenatalensisand leaf extract of eucomisautumnalis: carbohydrate metabolizing enzymes and pancreatic lipase inhibition:- Nwabuisi, Vincent C
ABSTRACT
Diabetes mellitus is a metabolic disorder of
glucose metabolism. The management of blood glucose level is the hallmark in
the treatment ofthis disease. This may be achieved through the use of oral
hypoglycemic drugs such as sulfonylureas, metformin, insulin secretagogues, and
alpha amylase, alpha glucosidase and pancreatic lipase inhibitors. The purpose
ofthe present study was to investigate the inliibitory effect of methanol bulb
extract of Bulbine natalensis and leaf extract of Eucomis autumnalis on the
activities of alpha amylase, alpha glucosidase and pancreatic lipase. This was
performed using porcine pancreatic alpha amylase from Aspergillus oryzae, alpha
glucosidase from Saccharomyces cerevisiae and pancreatic lipase. Highest
concentration of methanol bulb extract of B. natalensis and leaf extract of E.
autumnalis possesses the highest percentage inliibitory activity against
amylase (B.N Img/ml (70.15±2.14%), E.A Img/ml (71.19±0.85%), alpha glucosidase
(B.N 0.2mg/ml (73.24±1.05%), E.A 0.2mg/ml (62.1l±0.18%) and pancreatic lipase
(B.N Img/ml (49.69±2.37%), E.A Img/ml (38.44±2.86%) comparable to the standard
drugs, acarbose (80.34±0.18%) and orlistat (60.22±1.68%). Kinetic analysis
revealed that the methanol extract of both plants inhibited the alpha amylase
uncompetitively but displayed mixed noncompetitive mode of inhibition towards
alpha glucosidase and pancreatic lipase. It can be concluded that methanol
extracts of B. natalensis bulb and E. autumnalis leaves exhibited a potent
inhibitory activity on the three enzymes studied.
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MICHAEL, U (2024). Antidiabetic potential of methanol bulb extracts of bulbinenatalensisand leaf extract of eucomisautumnalis: carbohydrate metabolizing enzymes and pancreatic lipase inhibition:- Nwabuisi, Vincent C . Repository.mouau.edu.ng: Retrieved Nov 24, 2024, from https://repository.mouau.edu.ng/work/view/antidiabetic-potential-of-methanol-bulb-extracts-of-bulbinenatalensisand-leaf-extract-of-eucomisautumnalis-carbohydrate-metabolizing-enzymes-and-pancreatic-lipase-inhibition-nwabuisi-vincent-c-7-2
UNIVERSITY, MICHAEL. "Antidiabetic potential of methanol bulb extracts of bulbinenatalensisand leaf extract of eucomisautumnalis: carbohydrate metabolizing enzymes and pancreatic lipase inhibition:- Nwabuisi, Vincent C " Repository.mouau.edu.ng. Repository.mouau.edu.ng, 01 Mar. 2024, https://repository.mouau.edu.ng/work/view/antidiabetic-potential-of-methanol-bulb-extracts-of-bulbinenatalensisand-leaf-extract-of-eucomisautumnalis-carbohydrate-metabolizing-enzymes-and-pancreatic-lipase-inhibition-nwabuisi-vincent-c-7-2. Accessed 24 Nov. 2024.
UNIVERSITY, MICHAEL. "Antidiabetic potential of methanol bulb extracts of bulbinenatalensisand leaf extract of eucomisautumnalis: carbohydrate metabolizing enzymes and pancreatic lipase inhibition:- Nwabuisi, Vincent C ". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 01 Mar. 2024. Web. 24 Nov. 2024. < https://repository.mouau.edu.ng/work/view/antidiabetic-potential-of-methanol-bulb-extracts-of-bulbinenatalensisand-leaf-extract-of-eucomisautumnalis-carbohydrate-metabolizing-enzymes-and-pancreatic-lipase-inhibition-nwabuisi-vincent-c-7-2 >.
UNIVERSITY, MICHAEL. "Antidiabetic potential of methanol bulb extracts of bulbinenatalensisand leaf extract of eucomisautumnalis: carbohydrate metabolizing enzymes and pancreatic lipase inhibition:- Nwabuisi, Vincent C " Repository.mouau.edu.ng (2024). Accessed 24 Nov. 2024. https://repository.mouau.edu.ng/work/view/antidiabetic-potential-of-methanol-bulb-extracts-of-bulbinenatalensisand-leaf-extract-of-eucomisautumnalis-carbohydrate-metabolizing-enzymes-and-pancreatic-lipase-inhibition-nwabuisi-vincent-c-7-2