ABSTRACT

The antibiogram of organisms of public health importance from pork sold in Umuahia was investigated. Out of 30 samples collected from different selling points, the isolated bacteria species after microbial analysis were Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella spp and Proteus spp. The percentage occurrence of the microbial isolates were Staphyloccoccus aureus (40%),E.coli (33%), Pseudomonas aeruginosa (30%) Salmonella sp (17%) while Proteus sp (7%) showed the least occurrence. The antibiotic susceptibility tests of the isolates was done using the Kirby bauer disc diffusion method. The result of the antibiotics of sensitivity test showed that majority of the bacteria isolate was sensitive to the antibiotics. The isolates showed varying degrees of resistance on the antibiotics used. The isolates were highly susceptible to Streptomicin (68.4%) followed by Gentamicin (60.5%) and Ciprofloxacin (44.7%). The most resistant was recorded against Cefotaxime (81.6%), Augumentin (71.1%) and Oflaxacin (65.7%). The high resistance exhibited by the organisms in this study to various antibiotics has a great public health significance. 

TABLE OF CONTENTS

Title page i

Certification ii

Dedication iv

Acknowledgment v

Table of contents vi

List of Tables  viii

Abstract ix

CHAPTER ONE

1.0 Introduction 1 

1.1 Aim and Objectives 3

1.2 Objectives 3

CHAPTER TWO

2.0  Literature Review 4

2.1 Pork Meat 7

2.2 Haccp of Pork Meat 10

2.3 Pathogenic Organisms Associated With Pork Meat 12

2.4 Spoilage Microorganisms in Pork Meat 12

2.4.1Pathogens bacteria 14

CHAPTER THREE

3.0 Materials and Methods  17

3.1  Media Used 17

            3.2  Sterilization 17

3.3  Sample Preparation 17

3.4 Isolation and Identification of Bacterial Isolates 18

3.4.1 Isolation of Microorganisms 18

3.4.2 Identification of Microorganisms 18

3.5 Gram Staining 18

3.5.1 Motility Test 19

3.6  Biochemical Cultural Characteristics 19

3.6.1 Catalase Test 19

3.6.2 Coagulase Test 19

3.6.3 Citrate Test 20

3.6.4 Indole Test 20

3.6.5 Urease Test 20

3.6.6 Oxidase Test 21

3.6.7  Methyl Red 21

3.6.8 Voges Proskauer Test 21

3.6.9 Determination of Antimicrobial Susceptibility Pattern 22

CHAPTER FOUR

4.0 Results 23

CHAPTER FIVE

5.0 Discussion, Conclusion and Recommendation 29

5.1 Discussion 29

5.2 Conclusion 31

5.3 Recommendation  31

References 33