ABSTRACT

Six bacterial genera were isolated, which include; Pseudomonas aeruginosa, Bacillus sp, Micrococcus luteus, Escherichia coli, Proteus sp and Serratia marcasens. The total heterotrophic bacterial count showed that D1 had the highest count of 8.2 x 104 cfu/ml while F had the lowest count of 5.7 x 104 cfu/ml. The total coliform count showed that D1 had the highest coliform count of 6.9 x 104 cfu/ml, while CW1 had the lowest count of 4.4 x 104 cfu/ml. The percentage occurrence revealed that Escherichia coli had the highest frequency occurrence of 7 (28%) while Pseudomonas aeruginosa had the least frequency occurrence of 1 (4%). The antibiotic sensitivity revealed that all of the organisms are sensitive to some of the antibiotics used, the highest inhibitory activity of 22mm was recorded for Escherichia coli, Serratia marcasens, Proteus sp and Micrococcus luteus on Rifampicin, Ciprofloxacin, Gentamycin and Rifampicin respectively.

TABLE OF CONTENTS

Title Page i

Certification ii

Dedication iii

Acknowledgements iv

Table of Contents v

Lists of Tables vii

Abstract viii

CHAPTER ONE

1.0 Introduction 1

1.1 Aims and Objectives  3

1.2 Objectives 3

CHAPTER TWO 

2.0 Literature Review  4

2.1 Bacteria associated with Fish Pond 8

CHAPTER THREE 

3.0 Materials and Method 16

3.1 Sample Collection 16

3.2 Media Used 16

3.3 Sterilization  17

3.4 Bacterial count 17

3.5 Bacterial Isolation 17

3.6 Characterization and Identification of the Bacterial Isolates 17

3.6.1 Gram Staining 18

3.7 Biochemical Characteristics of the Isolates 18

3.7.1 Motility Test 18

3.7.2 Indole Test 19

3.7.3 Citrate Utilization 19

3.7.4 Sugar Fermentation 19

3.7.5 Oxidase Test 20

3.7.6 Coagulase Test 20

3.7.7 Urease Test 20

3.7.8 Methyl Red/ Voges Proskauer Test 21

3.8 Antibiotic Sensitivity Testing 21

CHAPTER FOUR

4.0  Results 22

CHAPTER FIVE

5.0       Discussion, Conclusion and Recommendation 28

5.1 Discussion 28

5.2 Conclusion 29

5.3 Recommendation 29

References 31