ABSTRACT

 The aim ofthis study is to determine antimicrobial activities of bitterleaf extract on fish pathogen. A total offifty 50 fresh fish samples were collected basically for this study. The fish sample were transported alive in plastic containers (covered with net) to the microbiology laboratory for analysis. The disease free bitterleaf was collected from National Root Crop Research Instituted (NRCRI) and was brought for identification and authentication at the Herbarium ofthe Department of Plant sciences and Biotechnology, Michael Okpara university of Agriculture, Umudike, Abia State. A total number of three (3) bacterial genera which belong to the genera Escherichia coli, Salmonella species and Staphylococcus aureus were isolated from fish. The 100% ,80%, 40% concentration ofmethanol extract ofbitterleaf shows higher zone ofinhibition more than the other concentration, on E. coli, S. aureus and Salmonella, 10% shows no inhibitory activity. The 100%, 80%, 40% and 20% concentration of ethanol extract shows higher zone of inhibition more than the other concentration, on E. coli, S. aureus and Salmonella, 10% shows no inhibitory activity. The 100% and 80% concentration of water extract shows higher zone of inhibition more than the other concentration, on E. coli, S. aureus and Salmonella, 20% and 10% shows no inhibitory activity. Shows the Phytochemicals present in aqueous extracts, methanol extract and ethanolic extracts were flavonoids, alkaloids, saponins, tannins and phenol.