ABSTRACT

 Anthocleista djalonensis has been found to have phytochemical properties and active ingredients of Endocrine modulatory activities. This study on Endocrine modulatory potentials of extracts of A. djalonensis leaves in experimentally stressed rats was carried out to investigate the potential effects of extracts ofA.djalonensis on Follicle stimulating hormone (FSH), Luteinizing hormone (LH), Estradiol (E2), Prolactin (PRL). 25 Female albino rats were randomly selected and grouped into five groups, A, B, C, D, and E respectively with A as control. Group B was given the standard drugs (Diazepam) while the rest C, D, and E were given the extracts at 200 and 400mg/kg body weight of crude and butanol fraction respectively. Both the standard drug and extracts were administered orally for 7days. Blood samples were collected from the rats using 5ml syringes dispersed into various test tubes and allowed for some time to clot. Clotted samples were centrifuged and Serum collected for further laboratory procedures. Serum concentration of the hormones were determined by ELISA method. The result of these study showed significant (P<0.05) reduction in all the tested groups compared to the standard and control experiments. Grouped D recorded the least PRL (1.26±0.37) followed by Group E (1.31±0.14) and then Group C (1.52±0.45). Group E recorded the least LH (5.41±0.12) followed by Group C (5.65±0.17) and then Group D (6.01±0.24). Group E recorded the least E2 (4.95±0.29) followed by Group D (5.22±0.01) and then Group C (5.30±0.76). Group C recorded the least FSH (3.24±0.19) followed by Group E (3.35±0.02) and then Group D (4.21±0.52) compared to the standard and control. The results of this study indicates metabolic stress due to reduced immune activities induced by the extracts. The results of this experiment show that this extract possesses negative effects on the female reproductive hormones and could not be used in the management of primary ovarian failure, irregular menstruation and miscarriage, though low prolactin is a positive fertility indicator.