Abstract
The
production and characterization of protease and pectinase from bacteria
isolated from market solid waste and the bio-scouring of cotton using the
enzymes was investigated. Using morphological, cultural and biochemical
properties, the isolate was confirmed as bacillus
subtilis. Protease and pectinase were produced by bacillus subtilis using
submerged fermentation in gelatin broth and pectin broth respectively. The
enzymes were purified using ammonium sulphate precipitation, dialysis and
ion-exchange chromatography. Glucose as a carbon source and peptone as a
nitrogen source gave optimum activity for both enzymes. Purified protease
exhibited maximum activity of 8.72u/ml at 40oc while pectinase
exhibited maximum activity of 8.98u/ml at 50oc. Application of the
enzymes on bio-scouring of cotton fabrics was tested. Pectinase was more
effective than protease under optimized conditions. Optimum scouring temperature
for both enzymes was between 40oc and 50oc with pectinase
bio-scoured fabric showing 15.5% weight loss while protease bio-scoured fabric
had 14.3% weight loss. Optimum ph for pectinase scouring was ph 9 with 14.8%
weight loss in fabric while optimum ph for protease scoured fabric was ph 7
with 12.3% weight loss in fabric. After 120 minutes of bio-scouring, 15.4%
weight loss was recorded for pectinase treated fabric while protease treated
fabric showed 10.9% weight loss. The application of protease and pectinase for
the scouring of cotton fabric revealed that they can be used as bio-scouring
agents for the treatment of textile materials.
ANAB-ATULOMAH, A (2023). Characterization And Bio-Scouring Potentials Of Protease And Pectinase Produced From Bacillus subtills Isolated From Waste. Repository.mouau.edu.ng: Retrieved Sep 08, 2024, from https://repository.mouau.edu.ng/work/view/characterization-and-bio-scouring-potentials-of-protease-and-pectinase-produced-from-bacillus-subtills-isolated-from-waste-7-2
ANAB-ATULOMAH, ANAB-ATULOMAH. "Characterization And Bio-Scouring Potentials Of Protease And Pectinase Produced From Bacillus subtills Isolated From Waste" Repository.mouau.edu.ng. Repository.mouau.edu.ng, 22 May. 2023, https://repository.mouau.edu.ng/work/view/characterization-and-bio-scouring-potentials-of-protease-and-pectinase-produced-from-bacillus-subtills-isolated-from-waste-7-2. Accessed 08 Sep. 2024.
ANAB-ATULOMAH, ANAB-ATULOMAH. "Characterization And Bio-Scouring Potentials Of Protease And Pectinase Produced From Bacillus subtills Isolated From Waste". Repository.mouau.edu.ng, Repository.mouau.edu.ng, 22 May. 2023. Web. 08 Sep. 2024. < https://repository.mouau.edu.ng/work/view/characterization-and-bio-scouring-potentials-of-protease-and-pectinase-produced-from-bacillus-subtills-isolated-from-waste-7-2 >.
ANAB-ATULOMAH, ANAB-ATULOMAH. "Characterization And Bio-Scouring Potentials Of Protease And Pectinase Produced From Bacillus subtills Isolated From Waste" Repository.mouau.edu.ng (2023). Accessed 08 Sep. 2024. https://repository.mouau.edu.ng/work/view/characterization-and-bio-scouring-potentials-of-protease-and-pectinase-produced-from-bacillus-subtills-isolated-from-waste-7-2