ABSTRACT

This research is designed to determine the antibacterial and antibiofilm activity of Zapotecca portoricensis roots, Artemisia annua leaves and Moringa oleifera seed oil.  To verify the uses, of root, leaf extracts and seed oil of these plants for medicinal purpose. They were screened for antibiofilm, antibacterial and antioxidant effects against some isolates which includes; Salmonella typhi, Staphylococcus aureus and Escherichia coli. Biofilm quantification and tissue culture method was used to determine the percentage biofilm inhibition. Agar well diffusion method was used to check the antibacterial activity of the various plant extracts, The lowest minimum inhibitory concentration (MIC) was done using the 2,3,5-triphenyl tetrazolium chloride (TTC).  Gas chromatography-mass spectroscopy (GC-MS) was used to identify bioactive compounds in these extract which include. 15.63 mg/ml was shown by Z. portoricensis and A. annua against E.coli. Extracts of A. annua leaves gave large inhibition zone diameter of 14mm against S aureus at 500 mg/ml. These extract gave high inhibition zone diameter of 12mm, 12.27mm and 14mm for M. oleifera, Z. portoricensis and A. annual respectively with % DPPH antioxidant activity of 32.50%, 45.24% and 73.25%. Maximum FRAP antioxidant activity and antibiofilm inhibition of extracts of M. oleifera, Z. portoricensis and A. annua observed were 1.02µm, 6.72µm and 0.54 µm for FRAP and 56.06%, 66.45% and 70.84% for % antibiofilm inhibition respectively. Results from this study indicate that extracts of M. oleifera, Z. portoricensis and A. annua contain bioactive compounds with possible potential as antibacterial, antioxidant and antibiofilm agents