Public health importance on hands of Microbiology Students of Michael Okpara University of Agriculture, Umudike

TABLE OF CONTENTS

Title page                                                                                                                                i

Certification                                                                                                                            ii

Dedication                                                                                                                              iii

Acknowledgement                                                                                                                  iv

Table of Contents                                                                                                                   v

List of Tables                                                                                                                          vii

                                                                                                                                    viii

Abstract                                                                                                                                  ix

CHAPTER ONE

1.1       Introduction                                                                                                                1

1.2       Aims and objectives                                                                                                    2

CHAPTER TWO

LITERATURE REVIEW

2. 1 Microbial ubiquity                                                                                                           3

2.2Skin microbiome                                                                                                                4

2.3       Hand contamination                                                                                                    5

2.4       Hand hygiene and personal protection                                                                       6

2.5 Characteristics of bacteria staphylococcus species                                                           7

CHAPTER THREE

MATERIALS AND METHOD

3.1 Sample collection                                                                                                              13

3.2 Sterilization of glass wares.                                                                                              13

3.3 Preparation of culture media                                                                                            13

3.4  Isolation of bacterial                                                                                                        14

3.5  Identification of bacterial                                                                                                 14

3.5.1 Gram staining                                                                                                                14

3.5.2Test of motility                                                                                                               14

3.5.3    Indole test                                                                                                                   15

3.5.4    Citrate test                                                                                                                  15

3.5.5    Catalase test                                                                                                                15 3.5.6 Coagulase test

3.5.7 Sugar fermentation test                                                                                                  15                                                                                                                                                        15       

CHAPTER FOUR

RESULTS

4.1 Prevalence of bacterial isolates                                                                                         17

CHAPTER FIVE

DISCUSSION, CONCLUSION AND RECOMMENDATION.

5.1       Discussion                                                                                                                   23

5.2       Conclusion                                                                                                                  24

5.3       Recommendation                                                                                                        24

References                                                                                                                             

LIST OF TABLES

Table                                      Title                                                                                        Page

 1:          Identification of bacterial isolates                                                                             18

2:           Carbohydrate utilization test                                                                                     19

3:           Distribution of bacterial isolate among the various level of students.                     20       

4:           Frequency of occurrence of the isolates among the various levels 

              of Microbiology students.                                                                                        21