Microbial Quality Of Frozen Fish Sold In Umuahia Metropolis

ABSTRACT

Microbial quality of frozen fishes obtained from three different markets were carried out using standard methods. The total viable count ranged from 1.2x104cfu/g to 1.9 x104cfu/g for Ndoro market, 1.4 x104cfu/g to 1.8 x104cfu/g for Orieugba and 1.2 x104cfu/g to 2.0 x104cfu/g for Ubani. Total fungi count showed their percentage occurrence of 6.6.7% (Ubani), 55.6% (Ndoro) and 55.6% (Orieugba). The bacteria isolated from fish samples were Staphylococcus, Proteus, Salmonella, Micrococcus, and E.coli specie, while fungi isolated were Aspergillus, Penicillum, and Yeasts. The study showed that the frozen fish samples were heavily contaminated which may be as result of poor sanitary practices employed by the vendors. This is of public health concern as these organisms are known causes of foodborne diseases.

TABLE OF CONTENTS

Title page ﾿ i

Certification ﾿ ii

Dedication ﾿ iii

Acknowledgements ﾿ iv

Table of contents ﾿ v

List of tables ﾿ vi

Abstract ﾿ vii

CHAPTER ONE

1.0 ﾿ INTRODUCTION ﾿ 1

1.2 ﾿ Aims and objectives ﾿ 4

CHAPTER TWO

2.0     LITERATURE REVIEW ﾿ 5

CHAPTER THREE

3.0 ﾿ MATERIALS AND METHODS ﾿ 9

3.1 ﾿ Source of materials ﾿ 9

3.2 ﾿ Sampling and sample preparation ﾿ 9

3.3 ﾿ Media preparation ﾿ 9

3.4 ﾿ Microbiological analysis ﾿ 10

3.5 ﾿ Determination of microbial flora ﾿ 11

3.5.1   Characterization of bacteria isolate ﾿ 11

3.5.1.1 Colony features  ﾿ 12

3.5.1.2 Microscopic features ﾿ 12

3.5.1.3 Biochemical reaction ﾿ 12

3.5.1.4 ﾿ Sugar utilization test ﾿ 12

3.5.2 ﾿ Characterization of fungi isolates ﾿ 12

3.5.2.1 Colony feature ﾿ 13

3.5.2.3 Structural features ﾿ 13

3.5.3 Identification of isolates ﾿ 13

3.5.4 ﾿ Determination of prevalence ﾿ 13

3.6. ﾿ Statistical analysis ﾿ 14

CHAPTER FOUR

4.0 ﾿ RESULTS ﾿ 15

CHAPTER FIVE

5.0 ﾿ DISCUSSION, RECOMMENDATION AND CONCLUSION ﾿ 20 ﾿

5.1 ﾿ Discussion ﾿ 20 ﾿

5.2 ﾿ Recommendation ﾿ 22

5.3 ﾿ Conclusion ﾿ 23

References ﾿ 24

      ﾿ Appendix ﾿