ISOLATION AND SCREENING OF ANTIBIOTIC-PRODUCING SOIL MICROORGANISMS AROUND FISH POND ENVIRONMENT IN UMUDIKE

ABSTRACT

The investigation was carried out to isolate antibiotics producing microorganisms around fish pond environment in Umudike. Soil samples were collected from 20 different locations of the fish pond for the isolation of potential antibiotic producing microorganisms. Spread plate technique was used for isolation and screening of microorganism. The media used for the isolation were Sabouraud dextrose agar, Tryptone soy agar and Nutrient agar. Sensitivity test using Staphylococcus aureus, Enterococcus feacalis, Pseudomonas aeruginosa, E.coli, as test organisms was employed  to investigate the antimicrobial activity of the isolates .Out of forty eight microorganisms  isolated, four of the isolates showed evidence of antimicrobial property against any of the bacteria test organisms and  they include; FPC1 which after biochemical screening was identified to be a Streptomyces species.,., FPA2 a Bacillus species., FPD2 a Bacillus species., and FPF3 a  Clostridium species., all the microbial isolates were inhibitory to the growth of atleast one of  the test bacteria . FPC1 produce inhibition zone of 9mm against P. areuginosa ,FPA2 produce inhibition zone of 8mm against  staphylococcus aureus ,  FPD2 produce inhibiton zone of 7mm  against E.faecalis and FPF3 produce inhibition of 6mm against P.aeruginosa. The result of this investigation demonstrated that strains of antibiotics producing microorganism are present in soil around fish pond environment  in  Umudike  and could be harnessed by pharmaceutical industries for the production of antibiotic from local sources. 

TABLE OF CONTENTS

CERTIFICATION ﾿ i                    

DEDICATION                                                                                                              ﾿ ii

ACKNOWLEDGEMENTS                                                                                          ﾿ iii

TABLE OF CONTENTS                                                                                              ﾿ iv

LIST OF TABLES                                                                                                        ﾿ vi

ABSTRACT                                                                                                                  ﾿ vii

CHAPTER ONE

1.0 ﾿ INTRODUCTION ﾿ 1

1.1 ﾿ Aims and Objectives                                                                                ﾿ 4

CHAPTER TWO

2.0 ﾿ Literature Review ﾿ 5       

2.1 ﾿ Factors affecting antibiotics production by microorganism                          ﾿ 7

2.2 ﾿ Past advances made in search for antibiotics producing 

              microorganisms in novel  environment                                                        ﾿ 11 

2.3 ﾿ Techniques employed in screening for antibiotics producing

             Microorganism ﾿ 15

CHAPTER THREE

﾿ Materials and Method ﾿ 18

3.0 ﾿ Sample Collection and Preparation ﾿ 18                                                            

3.1 ﾿ Media Preparation ﾿ 18

3.2 ﾿ Sample Preparation ﾿ 18

3.3 ﾿ Inoculation ﾿ 19

3.4 ﾿ Sub Culturing ﾿ 19

3.5 ﾿ Gram Staining ﾿ 19

3.6 ﾿ Biochemical Test ﾿ 20

3.6.1 ﾿ Hydrogen Sulfide test ﾿ 20

3.6.2 ﾿ Catalase Test ﾿ 20

3.6.3 ﾿ Starch Hydrolysis Test ﾿ 20

3.7 ﾿ Screening for Antimicrobial Activity ﾿ 20

CHAPTER FOUR

4.1 RESULT ﾿ 22

CHAPTER FIVE

5.0 Discussion ﾿ 26

5.1 Conclusion ﾿ 27

5.2 Recommendation ﾿ 28

 REFRENCE