ISOLATION AND IDENTIFICATION OF PROTEOLYTIC AND LIPOLYTIC MICROORGANISMS FROM SOIL RECEIVING ABATTOIR EFFLUENTS.

Abstract 

The isolation and identification of proteolytic and lipolytic microorganisms from soil receiving abattoir effluents were carried out. Soil samples contaminated with abattoir effluent were collected. The total aerobic plate count ranged from 1.1x107cfu/ml - 1.46x107cfu/ml; proteolytic count ranged from 1.01x106cfu/ml - 9.4x106cfu/ml; lipolytic count ranged from 0 to 6.1x106cfu/ml and fungal count ranged from 4.2x106cfu/ml - 6.6x106cfu/ml. The microorganisms isolated and their percentage occurrence were Staphylococcus aureus, 20%; Pseudomonas species,16%; Streptococcus species, 16%; Bacillus species, 14%; Micrococcus species, 6%; Escherichia coli, 12%; Klebseilla species, 16%; Aspergillus species, 57% and Rhizobus species, 42%. The results showed that the soil receiving abattoir effluent was contaminated heavily with known pathogenic microorganisms. The abattoir effluent should therefore be treated before discharge and good hygiene practices should also be maintained.

Table of contents

Title ﾿ i

Certification ﾿ ii

Dedication ﾿ iii

Acknowledgement ﾿ iv

Table of contents ﾿ v

List of tables ﾿ vii

Abstract ﾿ viii

Chapter One

1.1 ﾿ Introduction ﾿ 1

1.2 ﾿ Aim and Objectives ﾿ 5

Chapter Two

2.1 ﾿ Literature Review ﾿ 6

2.2 ﾿ Characteristics of proteases ﾿ 6

2.3 ﾿ Effect of Temperature on Protease and Lipases Production ﾿ 7

2.4 ﾿ Lipase producing Microorganisms ﾿ 7

2.5 ﾿ Bacteria Lipases ﾿ 8

2.6 ﾿ Sources of Lipases ﾿ 9

2.7 ﾿ Application of Microbial Lipases ﾿ 10

2.7.1 ﾿ Effluent Treatment ﾿ 10

2.7.2 ﾿ Medical Application ﾿ 11

2.7.3 ﾿ Lipases as a biosensor ﾿ 11

2.7.4 ﾿ Production of Biodegradable Polymers ﾿ 12

Chapter Three

3.0 ﾿ Materials and Methods ﾿ 13

3.1 ﾿ Study Site. ﾿ 13

3.2 ﾿ Collection of Samples ﾿ 13

3.3 ﾿ Chemical Reagents ﾿ 13

3.4 ﾿ Sterilization ﾿ 13

3.5 ﾿ Enumeration of Total Heterotrophic Bacteria and Fungi ﾿ 14

3.6 ﾿ Enumeration of Proteolytic and Lipolytic Bacteria. ﾿ 14

3.7 ﾿ Microbial Characterization/Identification ﾿ 15

3.7.1 ﾿ Gram Staining ﾿ 15

3.7.2 ﾿ Motility Test ﾿ 16

3.7.3 ﾿ Coagulase Test ﾿ 16

3.7.4 ﾿ Indole Test. ﾿ 16

3.7.5 ﾿ Catalase Test ﾿ 16

3.7.6 ﾿ Citrate Utilization ﾿ 17

3.7.7 ﾿ Oxidase Test. ﾿ 17

Chapter Four

4.1 ﾿ Results ﾿ 18

5.1 ﾿ Discussion ﾿ 23

5.2 ﾿ Conclusion ﾿ 25

5.3 ﾿ Recommendation ﾿ 25

References ﾿ 22

Appendix ﾿ 26

List of tables

Table ﾿ Title ﾿ Page

Table 1: The mean counts of the microorganism isolated from 

soil samples containing abattoir effluents ﾿ 19

Table 2: Microorganism isolated from soil abattoir effluent and 

their percentage occurrence ﾿ 20